Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan

The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection...

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Detalles Bibliográficos
Autores: Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R., Damonte, E.B.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Argentina
Institución:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
Repositorio:Biblioteca Digital (UBA-FCEN)
Idioma:inglés
OAI Identifier:paperaa:paper_00221317_v92_n6_p1332_Talarico
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico
Access Level:acceso abierto
Palabra clave:carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Mammalia
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oai_identifier_str paperaa:paper_00221317_v92_n6_p1332_Talarico
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenanTalarico, L.B.Noseda, M.D.Ducatti, D.R.B.Duarte, M.E.R.Damonte, E.B.carrageenanheparan sulfateanimal cellantiviral activityarticlecell proliferationcontrolled studydengueDengue virusinsect cellmammal cellmosquitononhumanpriority journalprotein synthesisVero cellvirus inhibitionAnimalsCarrageenanCell LineCercopithecus aethiopsCulicidaeDengueDengue VirusVero CellsVirus ReplicationDengue virusMammaliaThe antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_TalaricoJ. Gen. Virol. 2011;92(6):1332-1342reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2024-05-10T10:42:44Zpaperaa:paper_00221317_v92_n6_p1332_TalaricoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962024-05-10 10:42:45.551Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
spellingShingle Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
Talarico, L.B.
carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Dengue virus
Mammalia
title_short Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_full Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_fullStr Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_full_unstemmed Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_sort Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
dc.creator.none.fl_str_mv Talarico, L.B.
Noseda, M.D.
Ducatti, D.R.B.
Duarte, M.E.R.
Damonte, E.B.
author Talarico, L.B.
author_facet Talarico, L.B.
Noseda, M.D.
Ducatti, D.R.B.
Duarte, M.E.R.
Damonte, E.B.
author_role author
author2 Noseda, M.D.
Ducatti, D.R.B.
Duarte, M.E.R.
Damonte, E.B.
author2_role author
author
author
author
dc.subject.none.fl_str_mv carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Dengue virus
Mammalia
topic carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Dengue virus
Mammalia
description The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico
url http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv J. Gen. Virol. 2011;92(6):1332-1342
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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