Genomic relationships between Turnera orientalis and T. occidentalis (Turneraceae)

Aiming to differentiate Turnera orientalis (2n = 6x = 30) from T. occidentalis (2n = 6x = 30), traditional and molecular cytogenetic techniques were used. Numerous hybrids were obtained from controlled crosses between T. orientalis and T. occidentalis. An analysis of their pollen viability and the f...

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Detalles Bibliográficos
Autores: López, Alicia, Poggio, Lidia, Fernandez, Aveliano
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/69629
Acceso en línea:http://hdl.handle.net/11336/69629
Access Level:acceso abierto
Palabra clave:allopolyploid
fluorecent in situ hybridizatin
interspecific hybrids
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:Aiming to differentiate Turnera orientalis (2n = 6x = 30) from T. occidentalis (2n = 6x = 30), traditional and molecular cytogenetic techniques were used. Numerous hybrids were obtained from controlled crosses between T. orientalis and T. occidentalis. An analysis of their pollen viability and the failure to obtain F2 provided evidence of reproductive isolation. The present study reports for the first time the karyotype 22 metacentric + 8 submetacentric for T. occidentalis, and confirms the karyotype of T. orientalis. These species differ in a chromosome pair with secondary constriction and satellite. A FISH analysis showed six rDNA sites located on different chromosome pairs. Although the meiotic chromosome pairing of hybrids suggested a close relationship of the two species, the karyotype and mapped location of rDNA sites were different between the taxa, supporting that T. orientalis and T. occidentalis are distinct species.