Genomic relationships between Turnera orientalis and T. occidentalis (Turneraceae)

Aiming to differentiate Turnera orientalis (2n = 6x = 30) from T. occidentalis (2n = 6x = 30), traditional and molecular cytogenetic techniques were used. Numerous hybrids were obtained from controlled crosses between T. orientalis and T. occidentalis. An analysis of their pollen viability and the f...

ver descrição completa

Detalhes bibliográficos
Autores: López, Alicia, Poggio, Lidia, Fernandez, Aveliano
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2010
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/69629
Acesso em linha:http://hdl.handle.net/11336/69629
Access Level:Acceso aberto
Palavra-chave:allopolyploid
fluorecent in situ hybridizatin
interspecific hybrids
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descrição
Resumo:Aiming to differentiate Turnera orientalis (2n = 6x = 30) from T. occidentalis (2n = 6x = 30), traditional and molecular cytogenetic techniques were used. Numerous hybrids were obtained from controlled crosses between T. orientalis and T. occidentalis. An analysis of their pollen viability and the failure to obtain F2 provided evidence of reproductive isolation. The present study reports for the first time the karyotype 22 metacentric + 8 submetacentric for T. occidentalis, and confirms the karyotype of T. orientalis. These species differ in a chromosome pair with secondary constriction and satellite. A FISH analysis showed six rDNA sites located on different chromosome pairs. Although the meiotic chromosome pairing of hybrids suggested a close relationship of the two species, the karyotype and mapped location of rDNA sites were different between the taxa, supporting that T. orientalis and T. occidentalis are distinct species.