O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
A simple, eco friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β- hydroxyethyl)rutosid...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/5766 |
| Acceso en línea: | http://hdl.handle.net/11336/5766 |
| Access Level: | acceso abierto |
| Palabra clave: | O-(β-HYDROXYETHYL)RUTOSIDES MICELLAR ENHANCEMENT FLOW INJECTION SPECTROFLUORIMETRY PHARMACEUTICALS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | A simple, eco friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β- hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01 - 200 μg/mL with a detection limit of 0.001 μg/mL (s/n = 3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations. |
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