Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina

Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being <i>Fusarium graminearum</i> the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eigh...

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Detalles Bibliográficos
Autores: Consolo, Verónica Fabiana, Ortega, Leonel Maximiliano, Salerno, Graciela Lidia, Astoreca, Andrea Luciana, Alconada Magliano, Teresa María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/86718
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86718
Access Level:acceso abierto
Palabra clave:Ciencias Exactas
Fusarium graminearum
Fusarium Head Blight
Inter-simple sequence repeat
Intergenic spacer-restriction fragment length polymorphism
Wheat
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spelling Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of ArgentinaDiversidad genética de aislamientos de <i>Fusarium graminearum sensu lato</i> asociados con la fusariosis de la espiga en trigo de diversas localidades argentinasConsolo, Verónica FabianaOrtega, Leonel MaximilianoSalerno, Graciela LidiaAstoreca, Andrea LucianaAlconada Magliano, Teresa MaríaCiencias ExactasFusarium graminearumFusarium Head BlightInter-simple sequence repeatIntergenic spacer-restriction fragment length polymorphismWheatFusarium Head Blight is an important wheat disease in the Argentine Pampas region, being <i>Fusarium graminearum</i> the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 47 polymorphic bands. Both markers showed genetic variability among the analyzed isolates; however, IGS-RFLP was more efficient than ISSR, showing a higher polymorphic average (59.91%) than the latter (44.11%). The averages of polymorphic information content (PIC) were 0.211 and 0.129, respectively. Twenty haplotypes were identified by IGS-RFLP and 15 haplotypes by ISSR. Genotype clustering within dendrograms was different for both types of markers. The genetic groups obtained by IGS-RFLP showed a partial association to geographic origin. This is the first report on genetic variability of <i>F. graminearum</i> isolates from wheat in Argentina using IGS-RFLP and ISSR markers.La fusariosis de la espiga de trigo es una importante enfermedad para la región pampeana Argentina; <i>Fusarium graminearum</i> es el principal patógeno asociado. Se estudió el polimorfismo del ADN de un conjunto de aislamientos utilizando las técnicas de IGS-RFLP e ISSR. La técnica de IGS-RFLP produjo 41 bandas, 30 de ellas fueron polimórficas. El análisis de los ISSR mostró 87 bandas con 47 bandas polimórficas. La primera de estas metodologías fue más eficiente, ya que detectó mayor promedio polimórfico (59,91 %) que la segunda (44,11 %). Los valores promedio del contenido de información polimórfica (PIC) fueron 0,211 y 0,129, respectivamente. Se identificaron 20 haplotipos por IGS-RFLP, mientras que el análisis de los ISSR reveló 15 haplotipos. La agrupación de genotipos obtenida en ambos dendrogramas fue diferente. Los grupos genéticos obtenidos por la técnica de IGS-RFLP mostraron una asociación parcial con el origen geográfico. Este es el primer reporte que analiza la variabilidad genética en poblaciones de <i>F. graminearum</i> de trigo empleando marcadores IGS-RFLP e ISSR en Argentina.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf245-250http://sedici.unlp.edu.ar/handle/10915/86718enginfo:eu-repo/semantics/altIdentifier/issn/0325-7541info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2015.05.004info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:54:49Zoai:sedici.unlp.edu.ar:10915/86718Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:54:50.09SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
Diversidad genética de aislamientos de <i>Fusarium graminearum sensu lato</i> asociados con la fusariosis de la espiga en trigo de diversas localidades argentinas
title Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
spellingShingle Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
Consolo, Verónica Fabiana
Ciencias Exactas
Fusarium graminearum
Fusarium Head Blight
Inter-simple sequence repeat
Intergenic spacer-restriction fragment length polymorphism
Wheat
title_short Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
title_full Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
title_fullStr Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
title_full_unstemmed Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
title_sort Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
dc.creator.none.fl_str_mv Consolo, Verónica Fabiana
Ortega, Leonel Maximiliano
Salerno, Graciela Lidia
Astoreca, Andrea Luciana
Alconada Magliano, Teresa María
author Consolo, Verónica Fabiana
author_facet Consolo, Verónica Fabiana
Ortega, Leonel Maximiliano
Salerno, Graciela Lidia
Astoreca, Andrea Luciana
Alconada Magliano, Teresa María
author_role author
author2 Ortega, Leonel Maximiliano
Salerno, Graciela Lidia
Astoreca, Andrea Luciana
Alconada Magliano, Teresa María
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Fusarium graminearum
Fusarium Head Blight
Inter-simple sequence repeat
Intergenic spacer-restriction fragment length polymorphism
Wheat
topic Ciencias Exactas
Fusarium graminearum
Fusarium Head Blight
Inter-simple sequence repeat
Intergenic spacer-restriction fragment length polymorphism
Wheat
description Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being <i>Fusarium graminearum</i> the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 47 polymorphic bands. Both markers showed genetic variability among the analyzed isolates; however, IGS-RFLP was more efficient than ISSR, showing a higher polymorphic average (59.91%) than the latter (44.11%). The averages of polymorphic information content (PIC) were 0.211 and 0.129, respectively. Twenty haplotypes were identified by IGS-RFLP and 15 haplotypes by ISSR. Genotype clustering within dendrograms was different for both types of markers. The genetic groups obtained by IGS-RFLP showed a partial association to geographic origin. This is the first report on genetic variability of <i>F. graminearum</i> isolates from wheat in Argentina using IGS-RFLP and ISSR markers.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86718
url http://sedici.unlp.edu.ar/handle/10915/86718
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0325-7541
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2015.05.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
245-250
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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