Genetic diversity of <i>Fusarium graminearum sensu lato</i> isolates from wheat associated with Fusarium Head Blight in diverse geographic locations of Argentina
Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being <i>Fusarium graminearum</i> the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eigh...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/86718 |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/86718 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Exactas Fusarium graminearum Fusarium Head Blight Inter-simple sequence repeat Intergenic spacer-restriction fragment length polymorphism Wheat |
| Sumario: | Fusarium Head Blight is an important wheat disease in the Argentine Pampas region, being <i>Fusarium graminearum</i> the predominant pathogen. DNA polymorphism of the isolates was analyzed by IGS-RFLP and ISSR. IGS-RFLP and ISSR profiling were carried out using six endonucleases and eight primers, respectively. IGS-RFLP yielded 41 bands, 30 of which were polymorphic while ISSR produced 87 bands with 47 polymorphic bands. Both markers showed genetic variability among the analyzed isolates; however, IGS-RFLP was more efficient than ISSR, showing a higher polymorphic average (59.91%) than the latter (44.11%). The averages of polymorphic information content (PIC) were 0.211 and 0.129, respectively. Twenty haplotypes were identified by IGS-RFLP and 15 haplotypes by ISSR. Genotype clustering within dendrograms was different for both types of markers. The genetic groups obtained by IGS-RFLP showed a partial association to geographic origin. This is the first report on genetic variability of <i>F. graminearum</i> isolates from wheat in Argentina using IGS-RFLP and ISSR markers. |
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