New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides
The reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/156176 |
| Acceso en línea: | http://hdl.handle.net/11336/156176 |
| Access Level: | acceso abierto |
| Palabra clave: | 50,8-CYCLOPURINES 8-OXO-DG DNA DAMAGE FENTON REACTION FREE RADICALS GAMMA RADIOLYSIS OLIGONUCLEOTIDES PULSE RADIOLYSIS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | The reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides and ct-DNA in the range of 1 µs to 1 ms by pulse radiolysis using an Intensified Charged Coupled Device (ICCD) camera. Moreover, we applied our published analytical protocol based on an LC-MS/MS system with isotopomeric internal standards to enable accurate and precise measurements of purine lesion formation. In particular, the simultaneous measurement of the four purine 50,8-cyclo-20-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-20-deoxypurine (8-oxo-Pu) was obtained upon reaction of genetic material with HO• radicals generated either by γ-radiolysis or Fenton-type reactions. Our results contributed to the debate in the literature regarding absolute level of lesions, method of HO• radical generation, 50R/50S diastereomeric ratio in cPu, and relative abundance between cPu and 8-oxo-Pu. |
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