Full characterization of an IncR plasmid harboring qnrS1 recovered from a VIM-11-producing Pseudomonas aeruginosa
Introduction: Metallo-â-lactamases (MBL) producing Pseudomonas aeruginosa isolates have been well characterised. Quionolones are commonly used in the treatment of carbapenem resistant P. aeruginosa infections; however, data about PMQR in this species is scarce. The objective was to report the simult...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | español |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/135581 |
| Acceso en línea: | http://hdl.handle.net/11336/135581 |
| Access Level: | acceso abierto |
| Palabra clave: | METALLO-CARBAPENEMASE PMQR PSEUDOMONAS AERUGINOSA QNRS1-PLASMID https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
| Sumario: | Introduction: Metallo-â-lactamases (MBL) producing Pseudomonas aeruginosa isolates have been well characterised. Quionolones are commonly used in the treatment of carbapenem resistant P. aeruginosa infections; however, data about PMQR in this species is scarce. The objective was to report the simultaneous presence of qnrS and blaVIM-11 in P. aeruginosa, and to characterize the qnrS harbouring plasmid. Methods: Thirty-eight carbapenem resistant P. aeruginosa isolates were recovered at a hospital in Buenos Aires during 2012. Screening for MBL was assessed by double disc synergy test using EDTA and carbapenem discs. Plasmid DNA extraction was performed by a method using phenol44 chloroform. PCR followed by sequencing was carried out to determine each MBL and PMQR allele. PCR-BseGI-RFLP was performed to detect aac-(6?)-Ib-cr. The gyrA-QRDR was sequenced in those PMQR harbouring isolates. Plasmid incompatibility groups and addiction systems were characterised by PCR. The PMQR carrying plasmid was sequenced using Illumina technology, annotated using RAST and manually curated.Results: Eleven/38 isolates were VIM producers (blaVIM-2 and blaVIM-11) while 1/38 harboured blaIMP-13. One isolate harboured blaVIM-11 and the PMQR qnrS1, however, both markers were located in different plasmids. The qnrS1 harbouring plasmid (pP6qnrS1) was 117.945 bp in size, presented 154 CDS and corresponded to IncR group. In addition to qnrS1, it harboured several aminoglycoside resistancemarkers. Despite being pP6qnrS1 non-conjugative, it presented an OriT which makes it possible for this plasmid to be transferable.Conclusions: This is the first report on P. aeruginosa carrying both blaVIM-11 and qnrS1, plus the first detection of an IncR plasmid in Argentina. |
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