Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017

Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on...

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Autores: García, Nerea, Hernández, Marta, Gutierrez Boada, Maialen, Valero, Antonio, Navarro, Alejandro, Muñoz Chimeno, Milagros, Fernández Manzano, Alvaro, Escobar, Franco Matias, Martínez, Irene, Bárcena, Carmen, González, Sergio, Avellón, Ana, Eiros, Jose M., Fongaro, Gislaine, Domínguez, Lucas, Goyache, Joaquín, Rodríguez Lázaro, David
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/132427
Acesso em linha:http://hdl.handle.net/11336/132427
Access Level:acceso abierto
Palavra-chave:FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/132427
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
spellingShingle Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
García, Nerea
FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_full Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_fullStr Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_full_unstemmed Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
title_sort Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
dc.creator.none.fl_str_mv García, Nerea
Hernández, Marta
Gutierrez Boada, Maialen
Valero, Antonio
Navarro, Alejandro
Muñoz Chimeno, Milagros
Fernández Manzano, Alvaro
Escobar, Franco Matias
Martínez, Irene
Bárcena, Carmen
González, Sergio
Avellón, Ana
Eiros, Jose M.
Fongaro, Gislaine
Domínguez, Lucas
Goyache, Joaquín
Rodríguez Lázaro, David
author García, Nerea
author_facet García, Nerea
Hernández, Marta
Gutierrez Boada, Maialen
Valero, Antonio
Navarro, Alejandro
Muñoz Chimeno, Milagros
Fernández Manzano, Alvaro
Escobar, Franco Matias
Martínez, Irene
Bárcena, Carmen
González, Sergio
Avellón, Ana
Eiros, Jose M.
Fongaro, Gislaine
Domínguez, Lucas
Goyache, Joaquín
Rodríguez Lázaro, David
author_role author
author2 Hernández, Marta
Gutierrez Boada, Maialen
Valero, Antonio
Navarro, Alejandro
Muñoz Chimeno, Milagros
Fernández Manzano, Alvaro
Escobar, Franco Matias
Martínez, Irene
Bárcena, Carmen
González, Sergio
Avellón, Ana
Eiros, Jose M.
Fongaro, Gislaine
Domínguez, Lucas
Goyache, Joaquín
Rodríguez Lázaro, David
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic FOOD SAFETY
HEPATITIS E VIRUS
PIG ORGANS
PIGS
PORK CUTTINGS
PREVALENCE
SEROPREVALENCE
SLAUGHTERHOUSE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132427
García, Nerea; Hernández, Marta; Gutierrez Boada, Maialen; Valero, Antonio; Navarro, Alejandro; et al.; Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017; Frontiers Media S.A.; Frontiers in Microbiology; 10; 2990; 28-1-2020; 1-10
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132427
identifier_str_mv García, Nerea; Hernández, Marta; Gutierrez Boada, Maialen; Valero, Antonio; Navarro, Alejandro; et al.; Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017; Frontiers Media S.A.; Frontiers in Microbiology; 10; 2990; 28-1-2020; 1-10
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.02990/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02990
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195522462908416
spelling Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017García, NereaHernández, MartaGutierrez Boada, MaialenValero, AntonioNavarro, AlejandroMuñoz Chimeno, MilagrosFernández Manzano, AlvaroEscobar, Franco MatiasMartínez, IreneBárcena, CarmenGonzález, SergioAvellón, AnaEiros, Jose M.Fongaro, GislaineDomínguez, LucasGoyache, JoaquínRodríguez Lázaro, DavidFOOD SAFETYHEPATITIS E VIRUSPIG ORGANSPIGSPORK CUTTINGSPREVALENCESEROPREVALENCESLAUGHTERHOUSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.Fil: García, Nerea. Universidad Complutense de Madrid; EspañaFil: Hernández, Marta. Universidad de Burgos; EspañaFil: Gutierrez Boada, Maialen. Universidad de Burgos; EspañaFil: Valero, Antonio. Universidad de Córdoba; EspañaFil: Navarro, Alejandro. Universidad Complutense de Madrid; EspañaFil: Muñoz Chimeno, Milagros. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Fernández Manzano, Alvaro. Universidad Complutense de Madrid; EspañaFil: Escobar, Franco Matias. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Martínez, Irene. Universidad Complutense de Madrid; EspañaFil: Bárcena, Carmen. Universidad Complutense de Madrid; EspañaFil: González, Sergio. Universidad Complutense de Madrid; EspañaFil: Avellón, Ana. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Eiros, Jose M.. Hospital Universitario Rio Hortega; EspañaFil: Fongaro, Gislaine. Universidade Federal de Santa Catarina; BrasilFil: Domínguez, Lucas. Universidad Complutense de Madrid; EspañaFil: Goyache, Joaquín. Universidad Complutense de Madrid; EspañaFil: Rodríguez Lázaro, David. Universidad de Burgos; EspañaFrontiers Media S.A.2020-01-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132427García, Nerea; Hernández, Marta; Gutierrez Boada, Maialen; Valero, Antonio; Navarro, Alejandro; et al.; Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017; Frontiers Media S.A.; Frontiers in Microbiology; 10; 2990; 28-1-2020; 1-101664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.02990/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.02990info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:55:07Zoai:ri.conicet.gov.ar:11336/132427instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:55:07.882CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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