Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017

Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on...

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Detalles Bibliográficos
Autores: García Benzaquén, Nerea, Hernández, Marta, Gutierrez-Boada, Maialen, Valero, Antonio, Navarro Gómez, Alejandro, Muñoz-Chimeno, Milagros, Fernández Manzano, Álvaro, Escobar, Franco Matías, Martínez Alares, Irene, Bárcena Asensio, María Carmen, González Domínguez, Sergio, Avellón, Ana, Eiros, Jose María, Fongaro, Gislaine, Domínguez Rodríguez, Lucas José, Goyache Goñi, Joaquín, Rodríguez-Lázaro, David
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/93121
Acceso en línea:https://hdl.handle.net/20.500.14352/93121
Access Level:acceso abierto
Palabra clave:636.09
Food safety
Hepatitis E virus
Pig organs
Pigs
Pork cuttings
Prevalence
Seroprevalence
Slaughterhouse
Veterinaria
3109 Ciencias Veterinarias
Descripción
Sumario:Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.

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