Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice

Although many studies have explored the effects of acute or chronic ethanol exposure during the postimplantation period on embryo/fetal development, few reports have described the ethanol effects on preimplantation embryo development. Little is known about the effects of ethanol consumption prior to...

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Autores: Cebral, Elisa, Lasserre, Andrea, Besuhli, Valeria, Fernandez, Martha Alicia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/39226
Acceso en línea:http://hdl.handle.net/11336/39226
Access Level:acceso abierto
Palabra clave:Alcohol
Preimplantation
Mouse
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
title Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
spellingShingle Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
Cebral, Elisa
Alcohol
Preimplantation
Mouse
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
title_short Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
title_full Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
title_fullStr Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
title_full_unstemmed Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
title_sort Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice
dc.creator.none.fl_str_mv Cebral, Elisa
Lasserre, Andrea
Besuhli, Valeria
Fernandez, Martha Alicia
author Cebral, Elisa
author_facet Cebral, Elisa
Lasserre, Andrea
Besuhli, Valeria
Fernandez, Martha Alicia
author_role author
author2 Lasserre, Andrea
Besuhli, Valeria
Fernandez, Martha Alicia
author2_role author
author
author
dc.subject.none.fl_str_mv Alcohol
Preimplantation
Mouse
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
topic Alcohol
Preimplantation
Mouse
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
description Although many studies have explored the effects of acute or chronic ethanol exposure during the postimplantation period on embryo/fetal development, few reports have described the ethanol effects on preimplantation embryo development. Little is known about the effects of ethanol consumption prior to gestation on embryo growth. Recently, we have shown that chronic moderate ethanol intake by prepubertal female mice reduces the ovulatory response and impairs in vitro fertilization and in vitro embryo preimplantation development. The purpose of the present work was to evaluate the effects of preconceptional chronic moderate ethanol ingestion on preimplantation embryo morphology and differentiation, the timing of cleavage and embryo growth in vivo, and to determine the time pattern in which alterations appear. Prepubertal female mice were treated with 10% (w/v) ethanol for 30 days prior to conception. After inducing ovulation on day 27 and 29 of the ethanol treatment, females were mated with control males and the day of presence of vaginal plug was day 1. On day 1, a decreased percentage of normal fertilized oocytes, elevated parthenogenetic oocyte activation and unfertilized eggs with abnormal metaphase II were found in ethanol-treated, compared to control females. On day 2, while any differences in the total percentage of 2-cell embryos were observed, the treated females had a significantly higher percentage of morphologically abnormal embryos, compared to control females. On day 3, the preconceptional consumption of ethanol produced significantly reduced percentages of compacted morulae and an increased percentage of uncompacted morulae. The total percentage of morulae in the treated females was lower than in controls. On day 4, ethanol-treated females showed significantly decreased percentages of hatched attached blastocysts and increased early blastocyst and morula percentages, compared to controls. Thus, preconceptional chronic moderate ethanol ingestion by prepubertal female mice produced retarded development, impaired blastocyst hatching, abnormal embryo morphology and embryo loss by fragmentation due to alterations induced in the female gamete.
publishDate 2000
dc.date.none.fl_str_mv 2000-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39226
Cebral, Elisa; Lasserre, Andrea; Besuhli, Valeria; Fernandez, Martha Alicia; Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice; Oxford University Press; Alcohol and Alcoholism; 35; 4; 7-2000; 336-343
1464-3502
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39226
identifier_str_mv Cebral, Elisa; Lasserre, Andrea; Besuhli, Valeria; Fernandez, Martha Alicia; Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice; Oxford University Press; Alcohol and Alcoholism; 35; 4; 7-2000; 336-343
1464-3502
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/alcalc/35.4.336
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/alcalc/article/35/4/336/165112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female miceCebral, ElisaLasserre, AndreaBesuhli, ValeriaFernandez, Martha AliciaAlcoholPreimplantationMousehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Although many studies have explored the effects of acute or chronic ethanol exposure during the postimplantation period on embryo/fetal development, few reports have described the ethanol effects on preimplantation embryo development. Little is known about the effects of ethanol consumption prior to gestation on embryo growth. Recently, we have shown that chronic moderate ethanol intake by prepubertal female mice reduces the ovulatory response and impairs in vitro fertilization and in vitro embryo preimplantation development. The purpose of the present work was to evaluate the effects of preconceptional chronic moderate ethanol ingestion on preimplantation embryo morphology and differentiation, the timing of cleavage and embryo growth in vivo, and to determine the time pattern in which alterations appear. Prepubertal female mice were treated with 10% (w/v) ethanol for 30 days prior to conception. After inducing ovulation on day 27 and 29 of the ethanol treatment, females were mated with control males and the day of presence of vaginal plug was day 1. On day 1, a decreased percentage of normal fertilized oocytes, elevated parthenogenetic oocyte activation and unfertilized eggs with abnormal metaphase II were found in ethanol-treated, compared to control females. On day 2, while any differences in the total percentage of 2-cell embryos were observed, the treated females had a significantly higher percentage of morphologically abnormal embryos, compared to control females. On day 3, the preconceptional consumption of ethanol produced significantly reduced percentages of compacted morulae and an increased percentage of uncompacted morulae. The total percentage of morulae in the treated females was lower than in controls. On day 4, ethanol-treated females showed significantly decreased percentages of hatched attached blastocysts and increased early blastocyst and morula percentages, compared to controls. Thus, preconceptional chronic moderate ethanol ingestion by prepubertal female mice produced retarded development, impaired blastocyst hatching, abnormal embryo morphology and embryo loss by fragmentation due to alterations induced in the female gamete.Fil: Cebral, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Lasserre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Besuhli, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Fernandez, Martha Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaOxford University Press2000-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39226Cebral, Elisa; Lasserre, Andrea; Besuhli, Valeria; Fernandez, Martha Alicia; Alterations in preimplantation in vivo development after preconceptional chronic moderate alcohol consumption in female mice; Oxford University Press; Alcohol and Alcoholism; 35; 4; 7-2000; 336-3431464-3502CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/alcalc/35.4.336info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/alcalc/article/35/4/336/165112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:46:13Zoai:ri.conicet.gov.ar:11336/39226instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:46:13.319CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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