Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress

We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25°C) and in liquid crystalline states (50°C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does...

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Detalles Bibliográficos
Autores: Cutró, Andrea Carmen, Disalvo, Edgardo Anibal
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/196050
Acceso en línea:http://hdl.handle.net/11336/196050
Access Level:acceso abierto
Palabra clave:phenylalanine
merocyanine 540
DPPC - bilayers
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic StressCutró, Andrea CarmenDisalvo, Edgardo Anibalphenylalaninemerocyanine 540DPPC - bilayershttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25°C) and in liquid crystalline states (50°C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does not produce significant changes in MC 540 monomer concentration in DPPC LUVs at 50°C. In contrast, it significantly decreases the monomer adsorption in defects present in DPPC LUVs at 25°C. When DPPC LUVs were subjected to hypertonic stress, dehydration caused more defects, and in this case phenylalanine is also able to block such defects.Fil: Cutró, Andrea Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaAmerican Chemical Society2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196050Cutró, Andrea Carmen; Disalvo, Edgardo Anibal; Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress; American Chemical Society; Journal of Physical Chemistry B; 119; 31; 7-2015; 10060-100651520-6106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/acs.jpcb.5b05590info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jpcb.5b05590info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:58:54Zoai:ri.conicet.gov.ar:11336/196050instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:58:54.92CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
title Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
spellingShingle Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
Cutró, Andrea Carmen
phenylalanine
merocyanine 540
DPPC - bilayers
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
title_full Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
title_fullStr Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
title_full_unstemmed Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
title_sort Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
dc.creator.none.fl_str_mv Cutró, Andrea Carmen
Disalvo, Edgardo Anibal
author Cutró, Andrea Carmen
author_facet Cutró, Andrea Carmen
Disalvo, Edgardo Anibal
author_role author
author2 Disalvo, Edgardo Anibal
author2_role author
dc.subject.none.fl_str_mv phenylalanine
merocyanine 540
DPPC - bilayers
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic phenylalanine
merocyanine 540
DPPC - bilayers
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25°C) and in liquid crystalline states (50°C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does not produce significant changes in MC 540 monomer concentration in DPPC LUVs at 50°C. In contrast, it significantly decreases the monomer adsorption in defects present in DPPC LUVs at 25°C. When DPPC LUVs were subjected to hypertonic stress, dehydration caused more defects, and in this case phenylalanine is also able to block such defects.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196050
Cutró, Andrea Carmen; Disalvo, Edgardo Anibal; Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress; American Chemical Society; Journal of Physical Chemistry B; 119; 31; 7-2015; 10060-10065
1520-6106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196050
identifier_str_mv Cutró, Andrea Carmen; Disalvo, Edgardo Anibal; Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress; American Chemical Society; Journal of Physical Chemistry B; 119; 31; 7-2015; 10060-10065
1520-6106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/acs.jpcb.5b05590
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jpcb.5b05590
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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