Phenylalanine Blocks Defects Induced in Gel Lipid Membranes by Osmotic Stress
We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25°C) and in liquid crystalline states (50°C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/196050 |
| Acceso en línea: | http://hdl.handle.net/11336/196050 |
| Access Level: | acceso abierto |
| Palabra clave: | phenylalanine merocyanine 540 DPPC - bilayers https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25°C) and in liquid crystalline states (50°C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does not produce significant changes in MC 540 monomer concentration in DPPC LUVs at 50°C. In contrast, it significantly decreases the monomer adsorption in defects present in DPPC LUVs at 25°C. When DPPC LUVs were subjected to hypertonic stress, dehydration caused more defects, and in this case phenylalanine is also able to block such defects. |
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