Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis

Experimental design and optimization techniques were implemented for the development of a rapid and simple capillary zone electrophoresis method (CZE) for the determination of epinastine hydrochloride in human serum. The effects of five factors were studied on the resolution between the peaks for th...

ver descrição completa

Detalhes bibliográficos
Autores: Vera Candioti, Luciana, Olivieri, Alejandro Cesar, Goicoechea, Hector Casimiro
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2007
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/22817
Acesso em linha:http://hdl.handle.net/11336/22817
Access Level:Acceso aberto
Palavra-chave:Multiresponse Optimization
Capillary Electrophoresis
Epinastine
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
id AR_a8a6ef98eeecfd2ca567325b522a27bd
oai_identifier_str oai:ri.conicet.gov.ar:11336/22817
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
title Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
spellingShingle Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
Vera Candioti, Luciana
Multiresponse Optimization
Capillary Electrophoresis
Epinastine
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
title_full Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
title_fullStr Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
title_full_unstemmed Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
title_sort Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis
dc.creator.none.fl_str_mv Vera Candioti, Luciana
Olivieri, Alejandro Cesar
Goicoechea, Hector Casimiro
author Vera Candioti, Luciana
author_facet Vera Candioti, Luciana
Olivieri, Alejandro Cesar
Goicoechea, Hector Casimiro
author_role author
author2 Olivieri, Alejandro Cesar
Goicoechea, Hector Casimiro
author2_role author
author
dc.subject.none.fl_str_mv Multiresponse Optimization
Capillary Electrophoresis
Epinastine
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic Multiresponse Optimization
Capillary Electrophoresis
Epinastine
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description Experimental design and optimization techniques were implemented for the development of a rapid and simple capillary zone electrophoresis method (CZE) for the determination of epinastine hydrochloride in human serum. The effects of five factors were studied on the resolution between the peaks for the target analyte (epinastine hydrochloride) and lidocaine hydrochloride, used as internal standard, as well as on the analysis time.<br />The factors were the concentration and pH of the buffer, the injection time, the injection voltage and the separation voltage. The separation was carried out by using an uncoated silica capillary with 50 um i.d. and total length 64.5 cm (150 um of path length) and UV detection (200 nm).<br />Multiple response simultaneous optimization by using the desirability function was used to find experimental conditions where the system generates desirable results. The optimum conditions were: sodium phosphate buffer solution, 16.0 mmol/L; pH 8.50; injection voltage, 20.0 kV; injection time, 30 s; separation voltage, 26.7 kV.<br />The method was confirmed to be linear in the range of 2.0–12 ng/mL. The injection repeatability of the method was evaluated by six injections at three concentration levels, while intra-assay precision was assessed by analysing a single concentration level, yielding a CV’s of ca. 1% for standard and 2% for serum samples. Accuracy was evaluated by recovery assays and by comparing with an HPLC method, the results being acceptable according to regulatory agencies. The rudgeness was evaluated by means of an experimental Plackett–Burman design, in which the accuracy was assessed when small changes were set in the studied parameters. Clean-up of human serum samples was carried out by means of a liquid–liquid extraction procedure, which gave a high extraction yield for epinastine hydrochloride (93.00%).
publishDate 2007
dc.date.none.fl_str_mv 2007-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22817
Vera Candioti, Luciana; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis; Elsevier Science; Analytica Chimica Acta; 595; 1-2; 4-2007; 310-318
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22817
identifier_str_mv Vera Candioti, Luciana; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis; Elsevier Science; Analytica Chimica Acta; 595; 1-2; 4-2007; 310-318
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2007.04.032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195403056316416
spelling Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresisVera Candioti, LucianaOlivieri, Alejandro CesarGoicoechea, Hector CasimiroMultiresponse OptimizationCapillary ElectrophoresisEpinastinehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Experimental design and optimization techniques were implemented for the development of a rapid and simple capillary zone electrophoresis method (CZE) for the determination of epinastine hydrochloride in human serum. The effects of five factors were studied on the resolution between the peaks for the target analyte (epinastine hydrochloride) and lidocaine hydrochloride, used as internal standard, as well as on the analysis time.<br />The factors were the concentration and pH of the buffer, the injection time, the injection voltage and the separation voltage. The separation was carried out by using an uncoated silica capillary with 50 um i.d. and total length 64.5 cm (150 um of path length) and UV detection (200 nm).<br />Multiple response simultaneous optimization by using the desirability function was used to find experimental conditions where the system generates desirable results. The optimum conditions were: sodium phosphate buffer solution, 16.0 mmol/L; pH 8.50; injection voltage, 20.0 kV; injection time, 30 s; separation voltage, 26.7 kV.<br />The method was confirmed to be linear in the range of 2.0–12 ng/mL. The injection repeatability of the method was evaluated by six injections at three concentration levels, while intra-assay precision was assessed by analysing a single concentration level, yielding a CV’s of ca. 1% for standard and 2% for serum samples. Accuracy was evaluated by recovery assays and by comparing with an HPLC method, the results being acceptable according to regulatory agencies. The rudgeness was evaluated by means of an experimental Plackett–Burman design, in which the accuracy was assessed when small changes were set in the studied parameters. Clean-up of human serum samples was carried out by means of a liquid–liquid extraction procedure, which gave a high extraction yield for epinastine hydrochloride (93.00%).Fil: Vera Candioti, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Olivieri, Alejandro Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquimicas y Farmaceuticas. Departamento de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2007-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22817Vera Candioti, Luciana; Olivieri, Alejandro Cesar; Goicoechea, Hector Casimiro; Simultaneous multiresponse optimization applied to epinastine determination in human serum by using capillary electrophoresis; Elsevier Science; Analytica Chimica Acta; 595; 1-2; 4-2007; 310-3180003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2007.04.032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:51:28Zoai:ri.conicet.gov.ar:11336/22817instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:51:28.556CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15.811543

Registros relacionados