Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectromet...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/58580 |
| Acceso en línea: | http://hdl.handle.net/11336/58580 |
| Access Level: | acceso abierto |
| Palabra clave: | B12 Analogues Cobamide Corrinoids Lactic Acid Bacteria Lactobacillus Reuteri Pseudovitamin B12 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coα-[α-(7-adenyl)]-Coβ-cyanocobamide or pseudovitamin B12. This molecule differs from cobalamin in the α-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a α-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B12. © 2007 Federation of European Biochemical Societies. |
|---|