Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy

The aim of this chapter is to introduce an experimental approach that allows visualization of temperature-dependent lipid phase equilibria at the level of single vesicles, using two-photon fluorescence microscopy. Various methods to prepare giant unilamellar vesicles (GUVs) and novel results in the...

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Autor: Bagatolli, Luis Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/139218
Acceso en línea:http://hdl.handle.net/11336/139218
Access Level:acceso abierto
Palabra clave:GUVs
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence MicroscopyBagatolli, Luis AlbertoGUVshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this chapter is to introduce an experimental approach that allows visualization of temperature-dependent lipid phase equilibria at the level of single vesicles, using two-photon fluorescence microscopy. Various methods to prepare giant unilamellar vesicles (GUVs) and novel results in the direct visualization of the lipid phase coexistence at the level of single vesicles, combining the particular properties of some fluorescent probes and two-photon excitation fluorescence microscopy, are discussed in this chapter.Fil: Bagatolli, Luis Alberto. University Of Southern Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Academic Press Inc.2003-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139218Bagatolli, Luis Alberto; Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy; Elsevier Academic Press Inc.; Methods In Enzymology.; 367; 7-2003; 233-2530076-6879CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0076687903670151info:eu-repo/semantics/altIdentifier/doi/10.1016/S0076-6879(03)67015-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:47:17Zoai:ri.conicet.gov.ar:11336/139218instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:47:18.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
title Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
spellingShingle Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
Bagatolli, Luis Alberto
GUVs
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
title_full Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
title_fullStr Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
title_full_unstemmed Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
title_sort Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
dc.creator.none.fl_str_mv Bagatolli, Luis Alberto
author Bagatolli, Luis Alberto
author_facet Bagatolli, Luis Alberto
author_role author
dc.subject.none.fl_str_mv GUVs
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic GUVs
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The aim of this chapter is to introduce an experimental approach that allows visualization of temperature-dependent lipid phase equilibria at the level of single vesicles, using two-photon fluorescence microscopy. Various methods to prepare giant unilamellar vesicles (GUVs) and novel results in the direct visualization of the lipid phase coexistence at the level of single vesicles, combining the particular properties of some fluorescent probes and two-photon excitation fluorescence microscopy, are discussed in this chapter.
publishDate 2003
dc.date.none.fl_str_mv 2003-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139218
Bagatolli, Luis Alberto; Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy; Elsevier Academic Press Inc.; Methods In Enzymology.; 367; 7-2003; 233-253
0076-6879
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139218
identifier_str_mv Bagatolli, Luis Alberto; Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy; Elsevier Academic Press Inc.; Methods In Enzymology.; 367; 7-2003; 233-253
0076-6879
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0076687903670151
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0076-6879(03)67015-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Academic Press Inc.
publisher.none.fl_str_mv Elsevier Academic Press Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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