Thermotropic Behavior of Lipid Mixtures Studied at the Level of Single Vesicles: Giant Unilamellar Vesicles and Two-Photon Excitation Fluorescence Microscopy
The aim of this chapter is to introduce an experimental approach that allows visualization of temperature-dependent lipid phase equilibria at the level of single vesicles, using two-photon fluorescence microscopy. Various methods to prepare giant unilamellar vesicles (GUVs) and novel results in the...
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2003 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/139218 |
| Acceso en línea: | http://hdl.handle.net/11336/139218 |
| Access Level: | acceso abierto |
| Palabra clave: | GUVs https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | The aim of this chapter is to introduce an experimental approach that allows visualization of temperature-dependent lipid phase equilibria at the level of single vesicles, using two-photon fluorescence microscopy. Various methods to prepare giant unilamellar vesicles (GUVs) and novel results in the direct visualization of the lipid phase coexistence at the level of single vesicles, combining the particular properties of some fluorescent probes and two-photon excitation fluorescence microscopy, are discussed in this chapter. |
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