Impact of ancestral sequence reconstruction on mechanistic and structural enzymology

Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as...

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Authors: Nicoll, Callum R., Massari, Marta, Fraaije, Marco Wilhelmus, Mascotti, María Laura, Mattevi, Andrea
Format: article
Status:Published version
Publication Date:2023
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/227389
Online Access:http://hdl.handle.net/11336/227389
Access Level:Open access
Keyword:ANCESTRAL SEQUENCE RECONSTRUCTION
ENZYME BIOTECHNOLOGY
ENZYME EVOLUTION
MECHANISTIC ENZYMOLOGY
PROTEIN STABILITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227389
network_acronym_str AR
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repository_id_str
spelling Impact of ancestral sequence reconstruction on mechanistic and structural enzymologyNicoll, Callum R.Massari, MartaFraaije, Marco WilhelmusMascotti, María LauraMattevi, AndreaANCESTRAL SEQUENCE RECONSTRUCTIONENZYME BIOTECHNOLOGYENZYME EVOLUTIONMECHANISTIC ENZYMOLOGYPROTEIN STABILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide.Fil: Nicoll, Callum R.. Universita degli Studi di Pavia; ItaliaFil: Massari, Marta. Universita degli Studi di Pavia; ItaliaFil: Fraaije, Marco Wilhelmus. University of Groningen; Países BajosFil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. University of Groningen; Países BajosFil: Mattevi, Andrea. Universita degli Studi di Pavia; ItaliaElsevier2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227389Nicoll, Callum R.; Massari, Marta; Fraaije, Marco Wilhelmus; Mascotti, María Laura; Mattevi, Andrea; Impact of ancestral sequence reconstruction on mechanistic and structural enzymology; Elsevier; Current Opinion In Structural Biology; 82; 10-2023; 1-80959-440XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0959440X23001434info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbi.2023.102669info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:42:40Zoai:ri.conicet.gov.ar:11336/227389instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:42:41.07CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
title Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
spellingShingle Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
Nicoll, Callum R.
ANCESTRAL SEQUENCE RECONSTRUCTION
ENZYME BIOTECHNOLOGY
ENZYME EVOLUTION
MECHANISTIC ENZYMOLOGY
PROTEIN STABILITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
title_full Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
title_fullStr Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
title_full_unstemmed Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
title_sort Impact of ancestral sequence reconstruction on mechanistic and structural enzymology
dc.creator.none.fl_str_mv Nicoll, Callum R.
Massari, Marta
Fraaije, Marco Wilhelmus
Mascotti, María Laura
Mattevi, Andrea
author Nicoll, Callum R.
author_facet Nicoll, Callum R.
Massari, Marta
Fraaije, Marco Wilhelmus
Mascotti, María Laura
Mattevi, Andrea
author_role author
author2 Massari, Marta
Fraaije, Marco Wilhelmus
Mascotti, María Laura
Mattevi, Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANCESTRAL SEQUENCE RECONSTRUCTION
ENZYME BIOTECHNOLOGY
ENZYME EVOLUTION
MECHANISTIC ENZYMOLOGY
PROTEIN STABILITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic ANCESTRAL SEQUENCE RECONSTRUCTION
ENZYME BIOTECHNOLOGY
ENZYME EVOLUTION
MECHANISTIC ENZYMOLOGY
PROTEIN STABILITY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Ancestral sequence reconstruction (ASR) provides insight into the changes within a protein sequence across evolution. More specifically, it can illustrate how specific amino acid changes give rise to different phenotypes within a protein family. Over the last few decades it has established itself as a powerful technique for revealing molecular common denominators that govern enzyme function. Here, we describe the strength of ASR in unveiling catalytic mechanisms and emerging phenotypes for a range of different proteins, also highlighting biotechnological applications the methodology can provide.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227389
Nicoll, Callum R.; Massari, Marta; Fraaije, Marco Wilhelmus; Mascotti, María Laura; Mattevi, Andrea; Impact of ancestral sequence reconstruction on mechanistic and structural enzymology; Elsevier; Current Opinion In Structural Biology; 82; 10-2023; 1-8
0959-440X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227389
identifier_str_mv Nicoll, Callum R.; Massari, Marta; Fraaije, Marco Wilhelmus; Mascotti, María Laura; Mattevi, Andrea; Impact of ancestral sequence reconstruction on mechanistic and structural enzymology; Elsevier; Current Opinion In Structural Biology; 82; 10-2023; 1-8
0959-440X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0959440X23001434
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbi.2023.102669
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195066591346688
score 15,811543

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