A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification

Physiological levels of ROS support neurite outgrowth and axonal specification, but the mechanisms by which ROS are able to shape neurons remain unknown. Ca2 +, a broad intracellular second messenger, promotes both Rac1 activation and neurite extension. Ca2 + release from the endoplasmic reticulum,...

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Detalles Bibliográficos
Autores: Wilson Rodriguez, Carlos, Munoz-Palma, Ernesto, Henriquez, Daniel R., Palmisano, Ilaria, Nuñez, Marco Tulio, Di Giovanni, Simone, González Billault, Christian
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/72755
Acceso en línea:http://hdl.handle.net/11336/72755
Access Level:acceso abierto
Palabra clave:ACTIN CYTOSKELETON
AXON DEVELOPMENT
CALCIUM SIGNALING
NADPH OXIDASE
NEURONAL DIFFERENTIATION
REACTIVE OXYGEN SPECIES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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network_acronym_str AR
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repository_id_str
spelling A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specificationWilson Rodriguez, CarlosMunoz-Palma, ErnestoHenriquez, Daniel R.Palmisano, IlariaNuñez, Marco TulioDi Giovanni, SimoneGonzález Billault, ChristianACTIN CYTOSKELETONAXON DEVELOPMENTCALCIUM SIGNALINGNADPH OXIDASENEURONAL DIFFERENTIATIONREACTIVE OXYGEN SPECIEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Physiological levels of ROS support neurite outgrowth and axonal specification, but the mechanisms by which ROS are able to shape neurons remain unknown. Ca2 +, a broad intracellular second messenger, promotes both Rac1 activation and neurite extension. Ca2 + release from the endoplasmic reticulum, mediated by both the IP3R1 and ryanodine receptor (RyR) channels, requires physiological ROS levels that are mainly sustained by the NADPH oxidase (NOX) complex. In this work, we explore the contribution of the link between NOX and RyR-mediated Ca2 + release toward axonal specification of rat hippocampal neurons. Using genetic approaches, we find thatNOXactivation promotes both axonal development and Rac1 activation through a RyR-mediated mechanism, which in turn activates NOX through Rac1, one of the NOX subunits. Collectively, these data suggest a feedforward mechanism that integrates both NOX activity and RyR-mediated Ca2 + release to support cellular mechanisms involved in axon development.Fil: Wilson Rodriguez, Carlos. Universidad de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Munoz-Palma, Ernesto. Universidad de Chile; ChileFil: Henriquez, Daniel R.. Universidad de Chile; ChileFil: Palmisano, Ilaria. Imperial College London; Reino UnidoFil: Nuñez, Marco Tulio. Universidad de Chile; ChileFil: Di Giovanni, Simone. Imperial College London; Reino Unido. University of Tuebingen; AlemaniaFil: González Billault, Christian. Universidad de Chile; ChileSociety for Neuroscience2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72755Wilson Rodriguez, Carlos; Munoz-Palma, Ernesto; Henriquez, Daniel R.; Palmisano, Ilaria; Nuñez, Marco Tulio; et al.; A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification; Society for Neuroscience; Journal of Neuroscience; 36; 43; 10-2016; 11107-111190270-64741529-2401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/36/43/11107info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.1455-16.2016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:44:14Zoai:ri.conicet.gov.ar:11336/72755instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:44:14.887CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
title A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
spellingShingle A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
Wilson Rodriguez, Carlos
ACTIN CYTOSKELETON
AXON DEVELOPMENT
CALCIUM SIGNALING
NADPH OXIDASE
NEURONAL DIFFERENTIATION
REACTIVE OXYGEN SPECIES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
title_full A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
title_fullStr A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
title_full_unstemmed A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
title_sort A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification
dc.creator.none.fl_str_mv Wilson Rodriguez, Carlos
Munoz-Palma, Ernesto
Henriquez, Daniel R.
Palmisano, Ilaria
Nuñez, Marco Tulio
Di Giovanni, Simone
González Billault, Christian
author Wilson Rodriguez, Carlos
author_facet Wilson Rodriguez, Carlos
Munoz-Palma, Ernesto
Henriquez, Daniel R.
Palmisano, Ilaria
Nuñez, Marco Tulio
Di Giovanni, Simone
González Billault, Christian
author_role author
author2 Munoz-Palma, Ernesto
Henriquez, Daniel R.
Palmisano, Ilaria
Nuñez, Marco Tulio
Di Giovanni, Simone
González Billault, Christian
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ACTIN CYTOSKELETON
AXON DEVELOPMENT
CALCIUM SIGNALING
NADPH OXIDASE
NEURONAL DIFFERENTIATION
REACTIVE OXYGEN SPECIES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic ACTIN CYTOSKELETON
AXON DEVELOPMENT
CALCIUM SIGNALING
NADPH OXIDASE
NEURONAL DIFFERENTIATION
REACTIVE OXYGEN SPECIES
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Physiological levels of ROS support neurite outgrowth and axonal specification, but the mechanisms by which ROS are able to shape neurons remain unknown. Ca2 +, a broad intracellular second messenger, promotes both Rac1 activation and neurite extension. Ca2 + release from the endoplasmic reticulum, mediated by both the IP3R1 and ryanodine receptor (RyR) channels, requires physiological ROS levels that are mainly sustained by the NADPH oxidase (NOX) complex. In this work, we explore the contribution of the link between NOX and RyR-mediated Ca2 + release toward axonal specification of rat hippocampal neurons. Using genetic approaches, we find thatNOXactivation promotes both axonal development and Rac1 activation through a RyR-mediated mechanism, which in turn activates NOX through Rac1, one of the NOX subunits. Collectively, these data suggest a feedforward mechanism that integrates both NOX activity and RyR-mediated Ca2 + release to support cellular mechanisms involved in axon development.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72755
Wilson Rodriguez, Carlos; Munoz-Palma, Ernesto; Henriquez, Daniel R.; Palmisano, Ilaria; Nuñez, Marco Tulio; et al.; A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification; Society for Neuroscience; Journal of Neuroscience; 36; 43; 10-2016; 11107-11119
0270-6474
1529-2401
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72755
identifier_str_mv Wilson Rodriguez, Carlos; Munoz-Palma, Ernesto; Henriquez, Daniel R.; Palmisano, Ilaria; Nuñez, Marco Tulio; et al.; A feed-forward mechanism involving the NOX complex and RyR-mediated ca2 + release during axonal specification; Society for Neuroscience; Journal of Neuroscience; 36; 43; 10-2016; 11107-11119
0270-6474
1529-2401
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/36/43/11107
info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.1455-16.2016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,811543

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