Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 16...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 1986 |
| País: | Argentina |
| Recursos: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositório: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglês |
| OAI Identifier: | paperaa:paper_00219193_v168_n1_p270_Ugalde |
| Acesso em linha: | http://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_Ugalde |
| Access Level: | Acceso aberto |
| Palavra-chave: | galactose galacturonic acid polysaccharide uridine diphosphate galactose uridine diphosphate glucuronic acid article nonhuman priority journal Sinorhizobium meliloti Medicago sativa Rhizobium Scophthalmus rhombus |
| Resumo: | Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end. |
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