Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover

MicroRNAs are small regulatory RNAs involved in several processes in plants ranging from development and stress responses to defense against pathogens. In order to accomplish their molecular functions, miRNAs are methylated and loaded into one ARGONAUTE (AGO) protein, commonly known as AGO1, to stab...

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Autores: Giudicatti, Axel Joel, Tomassi, Ariel Hernán, Manavella, Pablo Andrés, Arce, Agustín Lucas
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/184539
Acceso en línea:http://hdl.handle.net/11336/184539
Access Level:acceso abierto
Palabra clave:ARABIDOPSIS THALIANA
ARGONAUTE 1
MIRNA TAILING
MIRNA TRIMMING
MIRNA TURNOVER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnoverGiudicatti, Axel JoelTomassi, Ariel HernánManavella, Pablo AndrésArce, Agustín LucasARABIDOPSIS THALIANAARGONAUTE 1MIRNA TAILINGMIRNA TRIMMINGMIRNA TURNOVERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1MicroRNAs are small regulatory RNAs involved in several processes in plants ranging from development and stress responses to defense against pathogens. In order to accomplish their molecular functions, miRNAs are methylated and loaded into one ARGONAUTE (AGO) protein, commonly known as AGO1, to stabilize and protect the molecule and to assemble a functional RNA-induced silencing complex (RISC). A specific machinery controls miRNA turnover to ensure the silencing release of targeted-genes in given circumstances. The trimming and tailing of miRNAs are fundamental modifications related to their turnover and, hence, to their action. In order to gain a better understanding of these modifications, we analyzed Arabidopsis thaliana small RNA sequencing data from a diversity of mutants, related to miRNA biogenesis, action, and turnover, and from different cellular fractions and immunoprecipitations. Besides confirming the effects of known players in these pathways, we found increased trimming and tailing in miRNA biogenesis mutants. More importantly, our analysis allowed us to reveal the importance of ARGONAUTE 1 (AGO1) loading, slicing activity, and cellular localization in trimming and tailing of miRNAs.Fil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Tomassi, Ariel Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaMDPI AG2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184539Giudicatti, Axel Joel; Tomassi, Ariel Hernán; Manavella, Pablo Andrés; Arce, Agustín Lucas; Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover; MDPI AG; Plants; 10; 2; 2-2021; 1-142223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/2/267info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10020267info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:47:26Zoai:ri.conicet.gov.ar:11336/184539instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:47:27.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
title Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
spellingShingle Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
Giudicatti, Axel Joel
ARABIDOPSIS THALIANA
ARGONAUTE 1
MIRNA TAILING
MIRNA TRIMMING
MIRNA TURNOVER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
title_full Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
title_fullStr Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
title_full_unstemmed Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
title_sort Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover
dc.creator.none.fl_str_mv Giudicatti, Axel Joel
Tomassi, Ariel Hernán
Manavella, Pablo Andrés
Arce, Agustín Lucas
author Giudicatti, Axel Joel
author_facet Giudicatti, Axel Joel
Tomassi, Ariel Hernán
Manavella, Pablo Andrés
Arce, Agustín Lucas
author_role author
author2 Tomassi, Ariel Hernán
Manavella, Pablo Andrés
Arce, Agustín Lucas
author2_role author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS THALIANA
ARGONAUTE 1
MIRNA TAILING
MIRNA TRIMMING
MIRNA TURNOVER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic ARABIDOPSIS THALIANA
ARGONAUTE 1
MIRNA TAILING
MIRNA TRIMMING
MIRNA TURNOVER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description MicroRNAs are small regulatory RNAs involved in several processes in plants ranging from development and stress responses to defense against pathogens. In order to accomplish their molecular functions, miRNAs are methylated and loaded into one ARGONAUTE (AGO) protein, commonly known as AGO1, to stabilize and protect the molecule and to assemble a functional RNA-induced silencing complex (RISC). A specific machinery controls miRNA turnover to ensure the silencing release of targeted-genes in given circumstances. The trimming and tailing of miRNAs are fundamental modifications related to their turnover and, hence, to their action. In order to gain a better understanding of these modifications, we analyzed Arabidopsis thaliana small RNA sequencing data from a diversity of mutants, related to miRNA biogenesis, action, and turnover, and from different cellular fractions and immunoprecipitations. Besides confirming the effects of known players in these pathways, we found increased trimming and tailing in miRNA biogenesis mutants. More importantly, our analysis allowed us to reveal the importance of ARGONAUTE 1 (AGO1) loading, slicing activity, and cellular localization in trimming and tailing of miRNAs.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184539
Giudicatti, Axel Joel; Tomassi, Ariel Hernán; Manavella, Pablo Andrés; Arce, Agustín Lucas; Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover; MDPI AG; Plants; 10; 2; 2-2021; 1-14
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184539
identifier_str_mv Giudicatti, Axel Joel; Tomassi, Ariel Hernán; Manavella, Pablo Andrés; Arce, Agustín Lucas; Extensive analysis of miRNA trimming and tailing indicates that AGO1 has a complex role in miRNA turnover; MDPI AG; Plants; 10; 2; 2-2021; 1-14
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/2/267
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10020267
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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