Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets

A capillary zone electrophoresis method for the simultaneous determination of pridinol mesylate (PRI) and meloxicam (MEL) employing epinastine hydrochloride and piroxicam as internal standards, was developed and optimized employing experimental design and response surface methodologies. The separati...

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Autores: Vignaduzzo, Silvana Edit, Vera Candioti, Luciana, Castellano, Patricia Margarita, Goicoechea, Hector Casimiro, Kaufman, Teodoro Saul
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/96499
Acceso en línea:http://hdl.handle.net/11336/96499
Access Level:acceso abierto
Palabra clave:CAPILLARY ZONE ELECTROPHORESIS
EXPERIMENTAL DESIGNS
MELOXICAM
PRIDINOL
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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spelling Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tabletsVignaduzzo, Silvana EditVera Candioti, LucianaCastellano, Patricia MargaritaGoicoechea, Hector CasimiroKaufman, Teodoro SaulCAPILLARY ZONE ELECTROPHORESISEXPERIMENTAL DESIGNSMELOXICAMPRIDINOLhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A capillary zone electrophoresis method for the simultaneous determination of pridinol mesylate (PRI) and meloxicam (MEL) employing epinastine hydrochloride and piroxicam as internal standards, was developed and optimized employing experimental design and response surface methodologies. The separation was optimally achieved in less than 2 min at 30 kV in an uncoated fused-silica capillary (41.4 cm × 75 μm I.D.), employing an 18 mmol L-1 sodium phosphate buffer solution (pH 5.90) at 25 °C. Samples were injected in hydrodynamic mode (50 mbar, 5 s) and the analytes were spectrophotometrically detected at 200 nm. Method robustness was demonstrated by ANOVA of determinations performed under conditions slightly different from the optimum. The method was validated regarding separation selectivity (peak purity factors > 0.99), linearity and range (PRI = 17.6-31.4 mg L-1; MEL = 66.5-122.5 mg L-1), accuracy (PRI = 100.2-101.9%; MEL = 98.9-100.7%) and precision. The RSD values obtained were ≥1.3% for injection repeatability and ≥1.9% for intra-day precision. The limits of detection (1.0 and 0.9 mg L-1) and quantification (3.3 and 16.5 mg L-1) of PRI and MEL, respectively, were also determined. The method was successfully applied to the determination of both drugs in three brands of tablet formulations. No statistically significant differences were observed when these results were compared with those of a RP-HPLC method.Fil: Vignaduzzo, Silvana Edit. Universidad Nacional de Rosario; ArgentinaFil: Vera Candioti, Luciana. National University Of The Litoral; ArgentinaFil: Castellano, Patricia Margarita. Universidad Nacional de Rosario; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; ArgentinaFil: Kaufman, Teodoro Saul. Universidad Nacional de Rosario; ArgentinaVieweg2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96499Vignaduzzo, Silvana Edit; Vera Candioti, Luciana; Castellano, Patricia Margarita; Goicoechea, Hector Casimiro; Kaufman, Teodoro Saul; Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets; Vieweg; Chromatographia; 74; 10-2011; 609-6170009-5893CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.springer.cominfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10337-011-2108-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:02:52Zoai:ri.conicet.gov.ar:11336/96499instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:02:52.97CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
title Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
spellingShingle Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
Vignaduzzo, Silvana Edit
CAPILLARY ZONE ELECTROPHORESIS
EXPERIMENTAL DESIGNS
MELOXICAM
PRIDINOL
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
title_full Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
title_fullStr Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
title_full_unstemmed Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
title_sort Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets
dc.creator.none.fl_str_mv Vignaduzzo, Silvana Edit
Vera Candioti, Luciana
Castellano, Patricia Margarita
Goicoechea, Hector Casimiro
Kaufman, Teodoro Saul
author Vignaduzzo, Silvana Edit
author_facet Vignaduzzo, Silvana Edit
Vera Candioti, Luciana
Castellano, Patricia Margarita
Goicoechea, Hector Casimiro
Kaufman, Teodoro Saul
author_role author
author2 Vera Candioti, Luciana
Castellano, Patricia Margarita
Goicoechea, Hector Casimiro
Kaufman, Teodoro Saul
author2_role author
author
author
author
dc.subject.none.fl_str_mv CAPILLARY ZONE ELECTROPHORESIS
EXPERIMENTAL DESIGNS
MELOXICAM
PRIDINOL
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic CAPILLARY ZONE ELECTROPHORESIS
EXPERIMENTAL DESIGNS
MELOXICAM
PRIDINOL
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description A capillary zone electrophoresis method for the simultaneous determination of pridinol mesylate (PRI) and meloxicam (MEL) employing epinastine hydrochloride and piroxicam as internal standards, was developed and optimized employing experimental design and response surface methodologies. The separation was optimally achieved in less than 2 min at 30 kV in an uncoated fused-silica capillary (41.4 cm × 75 μm I.D.), employing an 18 mmol L-1 sodium phosphate buffer solution (pH 5.90) at 25 °C. Samples were injected in hydrodynamic mode (50 mbar, 5 s) and the analytes were spectrophotometrically detected at 200 nm. Method robustness was demonstrated by ANOVA of determinations performed under conditions slightly different from the optimum. The method was validated regarding separation selectivity (peak purity factors > 0.99), linearity and range (PRI = 17.6-31.4 mg L-1; MEL = 66.5-122.5 mg L-1), accuracy (PRI = 100.2-101.9%; MEL = 98.9-100.7%) and precision. The RSD values obtained were ≥1.3% for injection repeatability and ≥1.9% for intra-day precision. The limits of detection (1.0 and 0.9 mg L-1) and quantification (3.3 and 16.5 mg L-1) of PRI and MEL, respectively, were also determined. The method was successfully applied to the determination of both drugs in three brands of tablet formulations. No statistically significant differences were observed when these results were compared with those of a RP-HPLC method.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96499
Vignaduzzo, Silvana Edit; Vera Candioti, Luciana; Castellano, Patricia Margarita; Goicoechea, Hector Casimiro; Kaufman, Teodoro Saul; Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets; Vieweg; Chromatographia; 74; 10-2011; 609-617
0009-5893
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96499
identifier_str_mv Vignaduzzo, Silvana Edit; Vera Candioti, Luciana; Castellano, Patricia Margarita; Goicoechea, Hector Casimiro; Kaufman, Teodoro Saul; Multivariate optimization and validation of a CZE method for the analysis of pridinol mesylate and meloxicam in tablets; Vieweg; Chromatographia; 74; 10-2011; 609-617
0009-5893
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.springer.com
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10337-011-2108-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Vieweg
publisher.none.fl_str_mv Vieweg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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