Development of diagnostic tools for IBDV detection using plants as bioreactors

Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been...

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Autores: Gomez, Evangelina Raquel, Cassani, María Florencia, Lucero, Maria Soledad, Parreño, Viviana, Chimeno Zoth, Silvina Andrea, Berinstein, Analia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Instituto Nacional de Tecnología Agropecuaria
Repositorio:INTA Digital (INTA)
Idioma:inglés
OAI Identifier:oai:localhost:20.500.12123/7766
Acceso en línea:http://hdl.handle.net/20.500.12123/7766
https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z
https://doi.org/10.1186/s13568-020-01029-z
Access Level:acceso abierto
Palabra clave:Enfermedades de los Animales
Virus Bursitis Infecciosa
Diagnóstico
Biorreactores
Animal Diseases
Infectious Bursal Disease Virus
Diagnosis
Bioreactors
Nicotiana benthamiana
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oai_identifier_str oai:localhost:20.500.12123/7766
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Development of diagnostic tools for IBDV detection using plants as bioreactors
title Development of diagnostic tools for IBDV detection using plants as bioreactors
spellingShingle Development of diagnostic tools for IBDV detection using plants as bioreactors
Gomez, Evangelina Raquel
Enfermedades de los Animales
Virus Bursitis Infecciosa
Diagnóstico
Biorreactores
Animal Diseases
Infectious Bursal Disease Virus
Diagnosis
Bioreactors
Nicotiana benthamiana
title_short Development of diagnostic tools for IBDV detection using plants as bioreactors
title_full Development of diagnostic tools for IBDV detection using plants as bioreactors
title_fullStr Development of diagnostic tools for IBDV detection using plants as bioreactors
title_full_unstemmed Development of diagnostic tools for IBDV detection using plants as bioreactors
title_sort Development of diagnostic tools for IBDV detection using plants as bioreactors
dc.creator.none.fl_str_mv Gomez, Evangelina Raquel
Cassani, María Florencia
Lucero, Maria Soledad
Parreño, Viviana
Chimeno Zoth, Silvina Andrea
Berinstein, Analia
author Gomez, Evangelina Raquel
author_facet Gomez, Evangelina Raquel
Cassani, María Florencia
Lucero, Maria Soledad
Parreño, Viviana
Chimeno Zoth, Silvina Andrea
Berinstein, Analia
author_role author
author2 Cassani, María Florencia
Lucero, Maria Soledad
Parreño, Viviana
Chimeno Zoth, Silvina Andrea
Berinstein, Analia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Virus Bursitis Infecciosa
Diagnóstico
Biorreactores
Animal Diseases
Infectious Bursal Disease Virus
Diagnosis
Bioreactors
Nicotiana benthamiana
topic Enfermedades de los Animales
Virus Bursitis Infecciosa
Diagnóstico
Biorreactores
Animal Diseases
Infectious Bursal Disease Virus
Diagnosis
Bioreactors
Nicotiana benthamiana
description Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-25T12:18:32Z
2020-08-25T12:18:32Z
2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7766
https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z
2191-0855
https://doi.org/10.1186/s13568-020-01029-z
url http://hdl.handle.net/20.500.12123/7766
https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z
https://doi.org/10.1186/s13568-020-01029-z
identifier_str_mv 2191-0855
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SpringerOpen
publisher.none.fl_str_mv SpringerOpen
dc.source.none.fl_str_mv AMB Express 10 : article number: 95 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
instname_str Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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spelling Development of diagnostic tools for IBDV detection using plants as bioreactorsGomez, Evangelina RaquelCassani, María FlorenciaLucero, Maria SoledadParreño, VivianaChimeno Zoth, Silvina AndreaBerinstein, AnaliaEnfermedades de los AnimalesVirus Bursitis InfecciosaDiagnósticoBiorreactoresAnimal DiseasesInfectious Bursal Disease VirusDiagnosisBioreactorsNicotiana benthamianaInfectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease.Instituto de BiotecnologíaFil: Gomez, Evangelina Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cassani, María Florencia. Universidad Nacional de Luján; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Lucero, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Berinstein, Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaSpringerOpen2020-08-25T12:18:32Z2020-08-25T12:18:32Z2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7766https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z2191-0855https://doi.org/10.1186/s13568-020-01029-zAMB Express 10 : article number: 95 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2024-05-15T12:24:38Zoai:localhost:20.500.12123/7766instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2024-05-15 12:24:39.124INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
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