Forensic analysis of dog (Canis lupus familiaris) mitochondrial DNA sequences: An inter-laboratory study of the GEP-ISFG working group

A voluntary collaborative exercise aiming at the mitochondrial analysis of canine biological samples was carried out in 2006-2008 by the Non-Human Forensic Genetics Commission of the Spanish and Portuguese Working Group (GEP) of the International Society for Forensic Genetics (ISFG). The participati...

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Detalhes bibliográficos
Autores: van Asch, Barbara, Albarran, Cristina, Alonso, Antonio, Angulo, Ramón, Alves, Cíntia, Betancor, Eva, Catanesi, Cecilia Ines, Corach, Daniel, Crespillo, Manuel, Doutremepuich, Christian, Estonba, Andone, Fernandes, Ana Teresa, Fernandez, Eugenia, Garcia, Ana Maria, Garcia, Miguel Angel, Gilardi, Patricia, Gonçalves, Rita, Hernández, Alexis, Lima, Gabriela, Nascimento, Eugênio, de Pancorbo, Marian M., Parra, David, Pinheiro, Maria de Fátima, Prat, Elena, Puente, Jorge, Ramírez, José Luis, Rendo, Fernando, Rey, Isabel, Di Rocco, Florencia, Rodríguez, Anayanci, Sala, Adriana Andrea, Salla, Joel, Sanchez, Juan J., Solá, Dácil, Silva, Sandra, Pestano Brito, José Juan, Amorim, António
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/138521
Acesso em linha:http://hdl.handle.net/11336/138521
Access Level:acceso abierto
Palavra-chave:CANIS LUPUS FAMILIARIS
COLLABORATIVE EXERCISE
GEP-ISFG
MTDNA
NON-HUMAN FORENSIC GENETICS
https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
Descrição
Resumo:A voluntary collaborative exercise aiming at the mitochondrial analysis of canine biological samples was carried out in 2006-2008 by the Non-Human Forensic Genetics Commission of the Spanish and Portuguese Working Group (GEP) of the International Society for Forensic Genetics (ISFG). The participating laboratories were asked to sequence two dog samples (one bloodstain and one hair sample) for the mitochondrial D-loop region comprised between positions 15,372 and 16,083 using suggested primers and PCR conditions, and to compare their results against a reference sequence. Twenty-one participating laboratories reported a total of 67.5% concordant results, 15% non-concordant results, and 17.5% no results. The hair sample analysis presented more difficulty to the participants than the bloodstain analysis, with a high percentage (29%) failing to obtain a result. The high level of participation showed the interest of the community in the analysis of dog forensic samples but the results reveal that crucial methodological issues need to be addressed and further training is required in order to respond proficiently to the demands of forensic casework.

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