Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics

In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density,...

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Detalhes bibliográficos
Autores: Hackenhaar, Camila Regina, Flores Rosa, Carolina, Esparza Flores, Elí Emanuel, Santagapita, Patricio Roman, Poletto Klein, Manuela, Hertz, Plinho Francisco
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/209624
Acesso em linha:http://hdl.handle.net/11336/209624
Access Level:Acceso aberto
Palavra-chave:ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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spelling Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristicsHackenhaar, Camila ReginaFlores Rosa, CarolinaEsparza Flores, Elí EmanuelSantagapita, Patricio RomanPoletto Klein, ManuelaHertz, Plinho FranciscoASPERGILLUS ORYZAEGELATINGENIPINIMMOBILIZATIONLACTOSE HYDROLYSISSODIUM ALGINATEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density, and interconnectivity of alginate rods) for a detailed analysis of the microstructure, as well as the thermal and operational stabilities of the enzyme. The structural modifications of the biocomposite determined by SAXS demonstrate that the addition of both genipin and enzyme produced a significant reduction in size and density of the Ca(II)-alginate rods. Immobilized β-galactosidase could be stored for 175 days under refrigeration maintaining 80% of its initial activity. Moreover, 90% of its relative activity was kept after 11 reuses in a batch process of lactose hydrolysis. Thus, the biocomposite proved to be effective as support for enzyme immobilization.Fil: Hackenhaar, Camila Regina. Universidade Federal do Rio Grande do Sul; BrasilFil: Flores Rosa, Carolina. Universidade Federal do Rio Grande do Sul; BrasilFil: Esparza Flores, Elí Emanuel. Universidade Federal do Rio Grande do Sul; BrasilFil: Santagapita, Patricio Roman. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Poletto Klein, Manuela. Universidade Federal de Ciencias Da Saúde de Porto Alegre; BrasilFil: Hertz, Plinho Francisco. Universidade Federal do Rio Grande do Sul; BrasilElsevier2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/209624Hackenhaar, Camila Regina; Flores Rosa, Carolina; Esparza Flores, Elí Emanuel; Santagapita, Patricio Roman; Poletto Klein, Manuela; et al.; Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics; Elsevier; Carbohydrate Polymers; 291; 9-2022; 1-370144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861722003885info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2022.119483info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:00:53Zoai:ri.conicet.gov.ar:11336/209624instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:00:54.19CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
spellingShingle Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
Hackenhaar, Camila Regina
ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_full Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_fullStr Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_full_unstemmed Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
title_sort Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics
dc.creator.none.fl_str_mv Hackenhaar, Camila Regina
Flores Rosa, Carolina
Esparza Flores, Elí Emanuel
Santagapita, Patricio Roman
Poletto Klein, Manuela
Hertz, Plinho Francisco
author Hackenhaar, Camila Regina
author_facet Hackenhaar, Camila Regina
Flores Rosa, Carolina
Esparza Flores, Elí Emanuel
Santagapita, Patricio Roman
Poletto Klein, Manuela
Hertz, Plinho Francisco
author_role author
author2 Flores Rosa, Carolina
Esparza Flores, Elí Emanuel
Santagapita, Patricio Roman
Poletto Klein, Manuela
Hertz, Plinho Francisco
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic ASPERGILLUS ORYZAE
GELATIN
GENIPIN
IMMOBILIZATION
LACTOSE HYDROLYSIS
SODIUM ALGINATE
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density, and interconnectivity of alginate rods) for a detailed analysis of the microstructure, as well as the thermal and operational stabilities of the enzyme. The structural modifications of the biocomposite determined by SAXS demonstrate that the addition of both genipin and enzyme produced a significant reduction in size and density of the Ca(II)-alginate rods. Immobilized β-galactosidase could be stored for 175 days under refrigeration maintaining 80% of its initial activity. Moreover, 90% of its relative activity was kept after 11 reuses in a batch process of lactose hydrolysis. Thus, the biocomposite proved to be effective as support for enzyme immobilization.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/209624
Hackenhaar, Camila Regina; Flores Rosa, Carolina; Esparza Flores, Elí Emanuel; Santagapita, Patricio Roman; Poletto Klein, Manuela; et al.; Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics; Elsevier; Carbohydrate Polymers; 291; 9-2022; 1-37
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/209624
identifier_str_mv Hackenhaar, Camila Regina; Flores Rosa, Carolina; Esparza Flores, Elí Emanuel; Santagapita, Patricio Roman; Poletto Klein, Manuela; et al.; Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics; Elsevier; Carbohydrate Polymers; 291; 9-2022; 1-37
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861722003885
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2022.119483
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.812429

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