Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides

Endosulfan (ES) modifies the ultrastructure of skeletal muscle fibers and causes changes to the swimming behavior of fish. The objectives of the present work were to evaluate, in fishes of Australoheros facetus, 1) the integrity of myofibrils (Mf) by the analysis of SDS-PAGE profiles, and 2) the fun...

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Detalhes bibliográficos
Autores: Crupkin, Andrea Carina, Iturburu, Fernando Gastón, Crupkin, Marcos, Menone, Mirta Lujan
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2018
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/88055
Acesso em linha:http://hdl.handle.net/11336/88055
Access Level:Acceso aberto
Palavra-chave:ENDOSULFAN
NUEVOS BIOMARCADORES
PECES
ECOTOXICIDAD
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
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network_acronym_str AR
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repository_id_str
spelling Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticidesCrupkin, Andrea CarinaIturburu, Fernando GastónCrupkin, MarcosMenone, Mirta LujanENDOSULFANNUEVOS BIOMARCADORESPECESECOTOXICIDADhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Endosulfan (ES) modifies the ultrastructure of skeletal muscle fibers and causes changes to the swimming behavior of fish. The objectives of the present work were to evaluate, in fishes of Australoheros facetus, 1) the integrity of myofibrils (Mf) by the analysis of SDS-PAGE profiles, and 2) the functionality of Mf through the microscopically monitoring of the contraction and changes in Mg2+ Ca2+- ATPase and Mg2+(EGTA) -ATPase activities. As expected, after the addition of the contraction buffer, control fish Mf contracted. On the contrary, Mf from fish exposed at 0.5 ug/L ES showed a partial contraction and none of the fish exposed at 10 ug/L ES contracted. As judged by its high Mg2+ Ca2+ ATPase activity and low Mg2+ (EGTA) ATPase activity, control Mf showed good functionality. In Mf from fish exposed to 0.5 and 10 ug/L ES the activities of these enzymes were similar, suggesting denaturation or degradation of some component of tropomyosin troponin complex. SDS-PAGE patterns of Mf from fish exposed to ES showed degradation of the myosin heavy chain and of tropomyosin. Similar values of lipid peroxidation (TBARS) were found in both control and exposed Mf, suggesting that lipid oxidation was not be related to the above-mentioned changes. The observed effects expand the knowledge of ES action in muscles and could be used as biomarkers of damage in fishes exposed to organochlorine compounds like the insecticide endosulfan.Fil: Crupkin, Andrea Carina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Crupkin, Marcos. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaAcademic Press Inc Elsevier Science2018-04-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88055Crupkin, Andrea Carina; Iturburu, Fernando Gastón; Crupkin, Marcos; Menone, Mirta Lujan; Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 158; 12-4-2018; 44-490147-65131090-2414CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0147651318302896info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.ecoenv.2018.03.096info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:23:06Zoai:ri.conicet.gov.ar:11336/88055instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:23:06.927CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
title Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
spellingShingle Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
Crupkin, Andrea Carina
ENDOSULFAN
NUEVOS BIOMARCADORES
PECES
ECOTOXICIDAD
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
title_short Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
title_full Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
title_fullStr Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
title_full_unstemmed Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
title_sort Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides
dc.creator.none.fl_str_mv Crupkin, Andrea Carina
Iturburu, Fernando Gastón
Crupkin, Marcos
Menone, Mirta Lujan
author Crupkin, Andrea Carina
author_facet Crupkin, Andrea Carina
Iturburu, Fernando Gastón
Crupkin, Marcos
Menone, Mirta Lujan
author_role author
author2 Iturburu, Fernando Gastón
Crupkin, Marcos
Menone, Mirta Lujan
author2_role author
author
author
dc.subject.none.fl_str_mv ENDOSULFAN
NUEVOS BIOMARCADORES
PECES
ECOTOXICIDAD
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
topic ENDOSULFAN
NUEVOS BIOMARCADORES
PECES
ECOTOXICIDAD
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
description Endosulfan (ES) modifies the ultrastructure of skeletal muscle fibers and causes changes to the swimming behavior of fish. The objectives of the present work were to evaluate, in fishes of Australoheros facetus, 1) the integrity of myofibrils (Mf) by the analysis of SDS-PAGE profiles, and 2) the functionality of Mf through the microscopically monitoring of the contraction and changes in Mg2+ Ca2+- ATPase and Mg2+(EGTA) -ATPase activities. As expected, after the addition of the contraction buffer, control fish Mf contracted. On the contrary, Mf from fish exposed at 0.5 ug/L ES showed a partial contraction and none of the fish exposed at 10 ug/L ES contracted. As judged by its high Mg2+ Ca2+ ATPase activity and low Mg2+ (EGTA) ATPase activity, control Mf showed good functionality. In Mf from fish exposed to 0.5 and 10 ug/L ES the activities of these enzymes were similar, suggesting denaturation or degradation of some component of tropomyosin troponin complex. SDS-PAGE patterns of Mf from fish exposed to ES showed degradation of the myosin heavy chain and of tropomyosin. Similar values of lipid peroxidation (TBARS) were found in both control and exposed Mf, suggesting that lipid oxidation was not be related to the above-mentioned changes. The observed effects expand the knowledge of ES action in muscles and could be used as biomarkers of damage in fishes exposed to organochlorine compounds like the insecticide endosulfan.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88055
Crupkin, Andrea Carina; Iturburu, Fernando Gastón; Crupkin, Marcos; Menone, Mirta Lujan; Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 158; 12-4-2018; 44-49
0147-6513
1090-2414
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88055
identifier_str_mv Crupkin, Andrea Carina; Iturburu, Fernando Gastón; Crupkin, Marcos; Menone, Mirta Lujan; Myofibrilar functional dysregulation in fish: A new biomarker of damage to pesticides; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 158; 12-4-2018; 44-49
0147-6513
1090-2414
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0147651318302896
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.ecoenv.2018.03.096
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812429

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