Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
Short-term responses at the antioxidant enzymatic systems, together with genotoxic effects were studied in the freshwater fish Australoheros facetus, exposed to endosulfan (ES) (0.02, 0.5, 5, 10 lg/L) for 24 h. Brain was the most responsive organ, showing inhibition of the enzymatic systems together...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/7964 |
| Acceso en línea: | http://hdl.handle.net/11336/7964 |
| Access Level: | acceso abierto |
| Palabra clave: | Antioxidant Enzymes Biomarkers Endosulfan Hydrogen Peroxide Lipid Peroxide Micronuclei https://purl.org/becyt/ford/1.5 https://purl.org/becyt/ford/1 |
| Sumario: | Short-term responses at the antioxidant enzymatic systems, together with genotoxic effects were studied in the freshwater fish Australoheros facetus, exposed to endosulfan (ES) (0.02, 0.5, 5, 10 lg/L) for 24 h. Brain was the most responsive organ, showing inhibition of the enzymatic systems together with an increase of hydrogen peroxide (H2O2) content. Concentration-dependent inhibition was observed for superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) with IC25 values of 0.012, 0.017, 0.018 lg/L, respectively. In liver, a similar behavior was observed for SOD with IC25 values of 2.22 lg/L. In addition, increased thiobarbituric acid reactive substances (TBARs) at 5 lg/L and H2O2 at 5 and 10 lg/L were observed. No effects were evidenced on ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), GR and CAT activities. In gills, only H2O2 decreased at 0.5 and 5 lg/L ES. Genotoxic effects were detected by the increase of the frequency of both, nuclear abnormalities (NA) at 0.02 lg/L and micronucleus (MN) at 5 lg/L. Environmentally realistic concentrations of ES exerted toxic responses in A. facetus, encouraging the further field validation of the observed pattern (tissue specificity, sensitiveness and concentration-response relationship) as a potential suit of biomarkers for assessing acute sublethal effects in A. facetus under short-term pulsed exposure to ES. |
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