Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method...
| Autores: | , , , , , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2008 |
| País: | Argentina |
| Recursos: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositório: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglês |
| OAI Identifier: | paperaa:paper_15306984_v8_n8_p2463_Bossi |
| Acesso em linha: | http://hdl.handle.net/20.500.12110/paper_15306984_v8_n8_p2463_Bossi |
| Access Level: | Acceso aberto |
| Palavra-chave: | Imaging techniques Mammals Molecular structure Optical switches Biological specimens Color channels Far fields Functional imaging Mammalian cells Molecular species Multicolor imaging New class Photoswitching Single-molecule levels Spatial resolutions Switchable Fluorescence fluorescent dye animal article cell line chemical structure chemistry color methodology microscopy Animals Cell Line Color Fluorescent Dyes Microscopy Molecular Structure |
| Resumo: | By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. © 2008 American Chemical Society. |
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