Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development

Fil: Rodríguez, M. B. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; Argentina

Detalles Bibliográficos
Autores: Rodríguez, M. B., Clérico, Gabriel José, Gambini, A., Rodríguez, D., Taminelli, Guillermo, Veronesi, J. C., Fernández, S., Sansiñena, Marina Julia, Salamone, D.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:Argentina
Institución:Pontificia Universidad Católica Argentina
Repositorio:Repositorio Institucional (UCA)
Idioma:inglés
OAI Identifier:oai:ucacris:123456789/9494
Acceso en línea:https://repositorio.uca.edu.ar/handle/123456789/9494
Access Level:acceso abierto
Palabra clave:FERTILIZACION IN VITRO
EQUINOS
OVOCITOS
DESARROLLO EMBRIONARIO
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oai_identifier_str oai:ucacris:123456789/9494
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
title Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
spellingShingle Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
Rodríguez, M. B.
FERTILIZACION IN VITRO
EQUINOS
OVOCITOS
DESARROLLO EMBRIONARIO
title_short Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
title_full Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
title_fullStr Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
title_full_unstemmed Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
title_sort Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development
dc.creator.none.fl_str_mv Rodríguez, M. B.
Clérico, Gabriel José
Gambini, A.
Rodríguez, D.
Taminelli, Guillermo
Veronesi, J. C.
Fernández, S.
Sansiñena, Marina Julia
Salamone, D.
author Rodríguez, M. B.
author_facet Rodríguez, M. B.
Clérico, Gabriel José
Gambini, A.
Rodríguez, D.
Taminelli, Guillermo
Veronesi, J. C.
Fernández, S.
Sansiñena, Marina Julia
Salamone, D.
author_role author
author2 Clérico, Gabriel José
Gambini, A.
Rodríguez, D.
Taminelli, Guillermo
Veronesi, J. C.
Fernández, S.
Sansiñena, Marina Julia
Salamone, D.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FERTILIZACION IN VITRO
EQUINOS
OVOCITOS
DESARROLLO EMBRIONARIO
topic FERTILIZACION IN VITRO
EQUINOS
OVOCITOS
DESARROLLO EMBRIONARIO
description Fil: Rodríguez, M. B. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; Argentina
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/9494
0737-0806
10.1016/j.jevs.2018.05.098
Rodríguez, M.B. et al. Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development [en línea]. Journal of Equine Veterinary Science. 2018, 66. doi:10.1016/j.jevs.2018.05.098 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9494
url https://repositorio.uca.edu.ar/handle/123456789/9494
identifier_str_mv 0737-0806
10.1016/j.jevs.2018.05.098
Rodríguez, M.B. et al. Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development [en línea]. Journal of Equine Veterinary Science. 2018, 66. doi:10.1016/j.jevs.2018.05.098 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9494
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Equine Veterinary Science. 2018, 66
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
instname_str Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
_version_ 1799197059354460160
spelling Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and developmentRodríguez, M. B.Clérico, Gabriel JoséGambini, A.Rodríguez, D.Taminelli, GuillermoVeronesi, J. C.Fernández, S.Sansiñena, Marina JuliaSalamone, D.FERTILIZACION IN VITROEQUINOSOVOCITOSDESARROLLO EMBRIONARIOFil: Rodríguez, M. B. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; ArgentinaFil: Rodríguez, M. B. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Clérico, Gabriel José. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción Animal; ArgentinaFil: Clérico, Gabriel José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gambini, A. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; ArgentinaFil: Gambini, A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Taminelli, Guillermo. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción Animal; ArgentinaFil: Veronesi, J. C. Frigorífico Equino Lamar; ArgentinaFil: Fernández, S. Frigorífico Equino Lamar; ArgentinaFil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción Animal; ArgentinaFil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salamone, D. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; ArgentinaFil: Salamone, D. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIntroduction: Demand for in vitro equine embryo production by ICSI has increased in clinical reproduction over the last 5 years. However, the efficiency of this technique remains variable with reported blastocyst rates between 10 and 41%. Oocytes recovered by transvaginal follicular aspiration from donor mares are valuable and limited, and they are often collected from immature follicles. These oocytes require further in vitro maturation in order to be competent to be fertilized and reach the blastocyst stage. For this reason, it is crucial to determine the optimal time to perform ICSI after onset of oocyte maturation. Different in vitro maturation intervals have been reported to produce ICSI embryos in horses (28-30h[1]; 36 h-[2]; 38 h [3]; 20-22 h- [4]). However, there is no consensus about the optimal maturation interval to produce high quality embryos. An important aspect of preimplantation embryo development is cell differentiation, with the formation of the inner cell mass (ICM) and the trophectoderm (TE). The Hippo signaling pathway has been shown to play an important role in mice and cattle [5,6], but it has not been described in horses to date. Nuclear localization of a protein called Yes-associated protein 1 (YAP1) in TE cells affects the expression of CDX2 and subsequent cell differentiation. YAP1 functions as a critical co-activator for TE development in the nucleus of TE cells, but in the inner cell mass, it stays mainly in the cytoplasm as a phosphorylated inactive form [6]. In this study, we aimed to evaluate developmental rates and quality of ICSI embryos produced from oocytes which exhibited extrusion of first polar body early in IVM (Group I, 22 to 24 h) or late (Group II, 28 to 30 h). Blastocyst development and size were recorded. Embryo quality was assesed by analysis of apoptotic cells (TUNEL assay), and expression and distribution of YAP-1 by immunoflourescence (IF).Elsevier2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/94940737-080610.1016/j.jevs.2018.05.098Rodríguez, M.B. et al. Effect of oocyte in vitro maturation interval on subsequent ICSI embryo quality and development [en línea]. Journal of Equine Veterinary Science. 2018, 66. doi:10.1016/j.jevs.2018.05.098 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9494Journal of Equine Veterinary Science. 2018, 66reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2024-05-15T12:24:32Zoai:ucacris:123456789/9494instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852024-05-15 12:24:32.574Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
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