The heavy chain variable segment gene repertoire in chronic Chagas' heart disease

Patients chronically infected with Trypanosoma cruzi develop chronic Chagas' heart disease (cChHD). Their Ab response is suspected to be involved in the cardiac pathogenesis. Reactivity of serum Abs from these patients has been extensively studied but little is known about the diversity of the...

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Autores: Grippo, Vanina, Mahler, Evelyn, Elias, Fernando E., Cauerhff, Ana, Gomez, Karina Andrea, Tentori, Maria C., Ruiz, Aurora, Vigliano, Carlos, Laguens, Rubén, Berek, Claudia, Levin, Mariano Jorge
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/79633
Acceso en línea:http://hdl.handle.net/11336/79633
Access Level:acceso abierto
Palabra clave:Chagas Disease
Immunoglobulin Heavy Chains
B-Lymphocytes
Gene Rearrangement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79633
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
title The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
spellingShingle The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
Grippo, Vanina
Chagas Disease
Immunoglobulin Heavy Chains
B-Lymphocytes
Gene Rearrangement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
title_full The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
title_fullStr The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
title_full_unstemmed The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
title_sort The heavy chain variable segment gene repertoire in chronic Chagas' heart disease
dc.creator.none.fl_str_mv Grippo, Vanina
Mahler, Evelyn
Elias, Fernando E.
Cauerhff, Ana
Gomez, Karina Andrea
Tentori, Maria C.
Ruiz, Aurora
Vigliano, Carlos
Laguens, Rubén
Berek, Claudia
Levin, Mariano Jorge
author Grippo, Vanina
author_facet Grippo, Vanina
Mahler, Evelyn
Elias, Fernando E.
Cauerhff, Ana
Gomez, Karina Andrea
Tentori, Maria C.
Ruiz, Aurora
Vigliano, Carlos
Laguens, Rubén
Berek, Claudia
Levin, Mariano Jorge
author_role author
author2 Mahler, Evelyn
Elias, Fernando E.
Cauerhff, Ana
Gomez, Karina Andrea
Tentori, Maria C.
Ruiz, Aurora
Vigliano, Carlos
Laguens, Rubén
Berek, Claudia
Levin, Mariano Jorge
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Immunoglobulin Heavy Chains
B-Lymphocytes
Gene Rearrangement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Chagas Disease
Immunoglobulin Heavy Chains
B-Lymphocytes
Gene Rearrangement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Patients chronically infected with Trypanosoma cruzi develop chronic Chagas' heart disease (cChHD). Their Ab response is suspected to be involved in the cardiac pathogenesis. Reactivity of serum Abs from these patients has been extensively studied but little is known about the diversity of the in vivo IgG repertoire. We analyzed 125 variable H chain (VH) genes and compared it to repertoires from healthy individuals, and patients with autoimmune processes and other infections. VH were from plasma cells isolated from heart tissue of three cChHD patients and from a Fab combinatorial library derived from bone marrow of another cChHD patient. The role of the parasite in shaping the Ab repertoire was assessed analyzing VH genes before and after panning against T. cruzi Ag. Among recovered VH genes, a significantly increased representation of VH4 was observed. Plasma cells at the site of cardiac infiltration showed an increased VH1 usage. CDR3 lengths were similar to the ones found in the healthy repertoire and significantly shorter than in other infections. VH derived from anti-T. cruzi Fab and plasma cells showed a higher proportion of hypermutated genes, 46.9% and 43.75%, respectively, vs 30.9% of the cChHD patient repertoire, pointing to the role of parasite Ags in the shaping of the humoral response in Chagas' disease. No histological evidence of germinal center-like structures was observed in heart tissue. In accordance, VH analysis of heart plasmocytes revealed no evidence of clonal B cell expansion, suggesting that they migrated into heart tissue from secondary lymphoid organs.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79633
Grippo, Vanina; Mahler, Evelyn; Elias, Fernando E.; Cauerhff, Ana; Gomez, Karina Andrea; et al.; The heavy chain variable segment gene repertoire in chronic Chagas' heart disease; American Association of Immunologists; Journal of Immunology; 183; 12; 12-2009; 8015-8025
0022-1767
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79633
identifier_str_mv Grippo, Vanina; Mahler, Evelyn; Elias, Fernando E.; Cauerhff, Ana; Gomez, Karina Andrea; et al.; The heavy chain variable segment gene repertoire in chronic Chagas' heart disease; American Association of Immunologists; Journal of Immunology; 183; 12; 12-2009; 8015-8025
0022-1767
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.0802375
info:eu-repo/semantics/altIdentifier/url/https://www.jimmunol.org/content/183/12/8015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196477752344576
spelling The heavy chain variable segment gene repertoire in chronic Chagas' heart diseaseGrippo, VaninaMahler, EvelynElias, Fernando E.Cauerhff, AnaGomez, Karina AndreaTentori, Maria C.Ruiz, AuroraVigliano, CarlosLaguens, RubénBerek, ClaudiaLevin, Mariano JorgeChagas DiseaseImmunoglobulin Heavy ChainsB-LymphocytesGene Rearrangementhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Patients chronically infected with Trypanosoma cruzi develop chronic Chagas' heart disease (cChHD). Their Ab response is suspected to be involved in the cardiac pathogenesis. Reactivity of serum Abs from these patients has been extensively studied but little is known about the diversity of the in vivo IgG repertoire. We analyzed 125 variable H chain (VH) genes and compared it to repertoires from healthy individuals, and patients with autoimmune processes and other infections. VH were from plasma cells isolated from heart tissue of three cChHD patients and from a Fab combinatorial library derived from bone marrow of another cChHD patient. The role of the parasite in shaping the Ab repertoire was assessed analyzing VH genes before and after panning against T. cruzi Ag. Among recovered VH genes, a significantly increased representation of VH4 was observed. Plasma cells at the site of cardiac infiltration showed an increased VH1 usage. CDR3 lengths were similar to the ones found in the healthy repertoire and significantly shorter than in other infections. VH derived from anti-T. cruzi Fab and plasma cells showed a higher proportion of hypermutated genes, 46.9% and 43.75%, respectively, vs 30.9% of the cChHD patient repertoire, pointing to the role of parasite Ags in the shaping of the humoral response in Chagas' disease. No histological evidence of germinal center-like structures was observed in heart tissue. In accordance, VH analysis of heart plasmocytes revealed no evidence of clonal B cell expansion, suggesting that they migrated into heart tissue from secondary lymphoid organs.Fil: Grippo, Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; ArgentinaFil: Mahler, Evelyn. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; ArgentinaFil: Elias, Fernando E.. Deutsches Rheumaforschungszentrum; AlemaniaFil: Cauerhff, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; ArgentinaFil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; ArgentinaFil: Tentori, Maria C.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Ruiz, Aurora. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Vigliano, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Favaloro; ArgentinaFil: Laguens, Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Favaloro; ArgentinaFil: Berek, Claudia. Deutsches Rheumaforschungszentrum; AlemaniaFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires; ArgentinaAmerican Association of Immunologists2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79633Grippo, Vanina; Mahler, Evelyn; Elias, Fernando E.; Cauerhff, Ana; Gomez, Karina Andrea; et al.; The heavy chain variable segment gene repertoire in chronic Chagas' heart disease; American Association of Immunologists; Journal of Immunology; 183; 12; 12-2009; 8015-80250022-1767CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.0802375info:eu-repo/semantics/altIdentifier/url/https://www.jimmunol.org/content/183/12/8015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:29:13Zoai:ri.conicet.gov.ar:11336/79633instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:29:13.681CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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