HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization

Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacryla...

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Detalles Bibliográficos
Autores: Torres, María Luz, Oberti, Tamara Gisela, Fernández, Juan Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/139939
Acceso en línea:http://hdl.handle.net/11336/139939
Access Level:acceso abierto
Palabra clave:SEMI-INTERPENETRATING POLYMER NETWORK
CHONDROCYTE
CHONDROCOMPATIBILITY
SWELLING
CONTACT ANGLE
https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
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repository_id_str
spelling HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterizationTorres, María LuzOberti, Tamara GiselaFernández, Juan ManuelSEMI-INTERPENETRATING POLYMER NETWORKCHONDROCYTECHONDROCOMPATIBILITYSWELLINGCONTACT ANGLEhttps://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE.Fil: Torres, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaFil: Oberti, Tamara Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Fernández, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación en Osteospatías y Metabolismo Mineral; ArgentinaTaylor & Francis2020-11-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139939Torres, María Luz; Oberti, Tamara Gisela; Fernández, Juan Manuel; HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization; Taylor & Francis; Journal of Biomaterials Science - Polymer Edition; 32; 4; 12-11-2020; 504-5230920-5063CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09205063.2020.1849920info:eu-repo/semantics/altIdentifier/doi/10.1080/09205063.2020.1849920info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:40:34Zoai:ri.conicet.gov.ar:11336/139939instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:40:34.392CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
title HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
spellingShingle HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
Torres, María Luz
SEMI-INTERPENETRATING POLYMER NETWORK
CHONDROCYTE
CHONDROCOMPATIBILITY
SWELLING
CONTACT ANGLE
https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
title_short HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
title_full HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
title_fullStr HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
title_full_unstemmed HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
title_sort HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization
dc.creator.none.fl_str_mv Torres, María Luz
Oberti, Tamara Gisela
Fernández, Juan Manuel
author Torres, María Luz
author_facet Torres, María Luz
Oberti, Tamara Gisela
Fernández, Juan Manuel
author_role author
author2 Oberti, Tamara Gisela
Fernández, Juan Manuel
author2_role author
author
dc.subject.none.fl_str_mv SEMI-INTERPENETRATING POLYMER NETWORK
CHONDROCYTE
CHONDROCOMPATIBILITY
SWELLING
CONTACT ANGLE
https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
topic SEMI-INTERPENETRATING POLYMER NETWORK
CHONDROCYTE
CHONDROCOMPATIBILITY
SWELLING
CONTACT ANGLE
https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
description Cartilage tissue engineering (CTE) has the general objective of restoring and improving damaged cartilage. A very interesting strategy of CTE is to combine different polymers to obtain a viscoelastic material. In the present study we have evaluated the applicability of Poly(2-hydroxyethyl methacrylate) networks semi-interpenetrated with sodium alginate for CTE. Alginate-containing hydrogels show an increase in scaffold porosity and swelling capacity, when compared with nonporous poly(2-hydroxyethyl methacrylate) scaffolds. Primary chondrocytes from young rats were cultured on the hydrogels, and an increase in chondrocyte proliferation and chondrocytic markers was observed in alginate-containing hydrogels. Chondrocytic phenotype was preserved on hydrogels containing the lowest amount of crosslinker and initiator (SEMI 3 and SEMI 4). In addition, Nitric oxide production by RAW264.7 macrophages grown on hydrogels was tested and none of the hydrogels showed high levels of this inflammatory marker after 2 days. These results indicate that our alginate-containing hydrogels could be useful for CTE.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139939
Torres, María Luz; Oberti, Tamara Gisela; Fernández, Juan Manuel; HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization; Taylor & Francis; Journal of Biomaterials Science - Polymer Edition; 32; 4; 12-11-2020; 504-523
0920-5063
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139939
identifier_str_mv Torres, María Luz; Oberti, Tamara Gisela; Fernández, Juan Manuel; HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: Synthesis and biological bharacterization; Taylor & Francis; Journal of Biomaterials Science - Polymer Edition; 32; 4; 12-11-2020; 504-523
0920-5063
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09205063.2020.1849920
info:eu-repo/semantics/altIdentifier/doi/10.1080/09205063.2020.1849920
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,811543

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