Micelles mediated separation fluorimetric methodology for Rhodamine B determination in condiments, snacks and candies

Dye Rhodamine B (RhB) has been widely used in textile industry and foodstuffs but due to its proved carcinogenicity and toxicity on human, many countries have forbidden its use in foods. A new methodology is proposed for Rhodamine B (RhB) fluorimetric determination applying separation with a surfact...

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Bibliographic Details
Authors: Alesso, Magdalena, Bondioli, Gabriel, Talio, María Carolina, Luconi, Marta Olga, Fernandez, Liliana Patricia
Format: article
Status:Published version
Publication Date:2012
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/93229
Online Access:http://hdl.handle.net/11336/93229
Access Level:Open access
Keyword:CLOUD POINT EXTRACTION
FOODS
RHODAMINE B
SPECTROFLUORIMETRY
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
Description
Summary:Dye Rhodamine B (RhB) has been widely used in textile industry and foodstuffs but due to its proved carcinogenicity and toxicity on human, many countries have forbidden its use in foods. A new methodology is proposed for Rhodamine B (RhB) fluorimetric determination applying separation with a surfactant. Experimental parameters that affect the separation and determination steps like surfactant concentration, time and temperature of equilibration, pH of extraction, nature and composition of diluting agent of surfactant rich phase have been studied and optimised. The implementation of separation step permitted to remove successfully the interferences of matrix components, improving selectivity and sensitivity. Developed methodology presents a linearity range between 4.67 × 10 -2 and 100 μg L -1 with a correlation coefficient of 0.999. At optimal experimental conditions, a LOD of 1.40 × 10 -2 μg L -1 and LOQ of 4.67 × 10 -2 μg L -1 were obtained, allowing RhB determination in a variety of commercial condiments, snacks and bubble gums.

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