Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: involvement of lipid rafts
The aim of the present study was to evaluate the capacity of Lactobacillus reuteri CRL1098 soluble factors (Lr-S) to modulate TNF-α production in peripheral blood mononuclear cells (PBMC) and to study lipid rafts participation in this response. PBMC treated with Lr-S showed a reduced production of T...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/25073 |
| Acceso en línea: | http://hdl.handle.net/11336/25073 |
| Access Level: | acceso abierto |
| Palabra clave: | Lactobacillus Reuteri Crl 1098 Lipid Rafts Pbmc Tnf-Α Secreted Peptide https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
| Sumario: | The aim of the present study was to evaluate the capacity of Lactobacillus reuteri CRL1098 soluble factors (Lr-S) to modulate TNF-α production in peripheral blood mononuclear cells (PBMC) and to study lipid rafts participation in this response. PBMC treated with Lr-S showed a reduced production of TNF-α. In addition, Lr-S treatment activated ERK and p38 MAPK pathways in PBMC. Lipid rafts participation in the reduced production of TNF-α by PBMC induced by Lr-S was verified by lipid rafts disruption with methyl-β-cyclodextrin and the reduction of the Src-tyrosine kinase Lck localization in rafts. Moreover, PBMC pre-treatment with Lck inhibitors blocked the effect of Lr-S on TNF-α production suggesting that activation and mobilization of Lck from lipid rafts would be involved in the modulatory effect of L. reuteri CRL1098. A secreted peptide of 5785 Da would be responsible of the modulatory effect of CRL1098 strain. This study demonstrated for the first time the lipid rafts participation in a response induced by a beneficial bacterium. Also, these results open new possibilities for investigating the molecular mechanisms involved in the interaction of probiotic bacterial extracellular compounds with immune cells. |
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