Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.

Abstract:Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livest...

Descripción completa

Detalles Bibliográficos
Autores: CASTELLS, M., CAFFARENA, D., CASAUX, M.L., SCHILD, C., MIÑO, S., CASTELLS, F., CASTELLS, D., VICTORIA, M., RIET-CORREA, F., GIANNITTI, F., PAREÑO, V., COLINA, R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Uruguay
Institución:Instituto Nacional de Investigación Agropecuaria
Repositorio:AINFO
Idioma:inglés
OAI Identifier:oai:redi.anii.org.uy:20.500.12381/4103
Acceso en línea:https://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250
Access Level:acceso abierto
Palabra clave:PLATAFORMA SALUD ANIMAL
ROTAVIRUS
BOVINE
GENOTYPES
INTERSPECIES TRANSMISSION
DIARRHEA
GANADO BOVINO
id UY_e67fef02ab585db747c6e41e577a024b
oai_identifier_str oai:redi.anii.org.uy:20.500.12381/4103
network_acronym_str UY
network_name_str Uruguay
repository_id_str
spelling 2025-06-23T18:20:21Z2025-06-23T18:20:21Z20202025-06-23T18:20:21Zhttps://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250Abstract:Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.https://hdl.handle.net/20.500.12381/4103enenginfo:eu-repo/semantics/openAccessAcceso abiertoPLATAFORMA SALUD ANIMALROTAVIRUSBOVINEGENOTYPESINTERSPECIES TRANSMISSIONDIARRHEAGANADO BOVINOPhylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.ArticlePublishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:AINFOinstname:Instituto Nacional de Investigación Agropecuariainstacron:Instituto Nacional de Investigación AgropecuariaCASTELLS, M.CAFFARENA, D.CASAUX, M.L.SCHILD, C.MIÑO, S.CASTELLS, F.CASTELLS, D.VICTORIA, M.RIET-CORREA, F.GIANNITTI, F.PAREÑO, V.COLINA, R.SWORDsword-2025-06-23T15:20:21.original.xmlOriginal SWORD entry documentapplication/octet-stream3245https://redi.anii.org.uy/jspui/bitstream/20.500.12381/4103/1/sword-2025-06-23T15%3a20%3a21.original.xml9ed3751005100bcb6f97039adbf031bcMD5120.500.12381/41032026-02-10 15:53:55.404oai:redi.anii.org.uy:20.500.12381/4103Institucionalhttps://ainfo.inia.uy/Organismo científico-tecnológicohttp://inia.uyhttps://redi.anii.org.uy/oai/requestlorrego@inia.org.uyUruguayopendoar:2026-02-10T18:53:55AINFO - Instituto Nacional de Investigación Agropecuariafalse
dc.title.none.fl_str_mv Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
title Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
spellingShingle Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
CASTELLS, M.
PLATAFORMA SALUD ANIMAL
ROTAVIRUS
BOVINE
GENOTYPES
INTERSPECIES TRANSMISSION
DIARRHEA
GANADO BOVINO
title_short Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
title_full Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
title_fullStr Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
title_full_unstemmed Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
title_sort Phylogenetic analyses of rotavirus a from cattle in uruguay reveal the circulation of common and uncommon genotypes and suggest interspecies transmisión.
dc.creator.none.fl_str_mv CASTELLS, M.
CAFFARENA, D.
CASAUX, M.L.
SCHILD, C.
MIÑO, S.
CASTELLS, F.
CASTELLS, D.
VICTORIA, M.
RIET-CORREA, F.
GIANNITTI, F.
PAREÑO, V.
COLINA, R.
author CASTELLS, M.
author_facet CASTELLS, M.
CAFFARENA, D.
CASAUX, M.L.
SCHILD, C.
MIÑO, S.
CASTELLS, F.
CASTELLS, D.
VICTORIA, M.
RIET-CORREA, F.
GIANNITTI, F.
PAREÑO, V.
COLINA, R.
author_role author
author2 CAFFARENA, D.
CASAUX, M.L.
SCHILD, C.
MIÑO, S.
CASTELLS, F.
CASTELLS, D.
VICTORIA, M.
RIET-CORREA, F.
GIANNITTI, F.
PAREÑO, V.
COLINA, R.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLATAFORMA SALUD ANIMAL
ROTAVIRUS
BOVINE
GENOTYPES
INTERSPECIES TRANSMISSION
DIARRHEA
GANADO BOVINO
topic PLATAFORMA SALUD ANIMAL
ROTAVIRUS
BOVINE
GENOTYPES
INTERSPECIES TRANSMISSION
DIARRHEA
GANADO BOVINO
description Abstract:Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.
publishDate 2020
dc.date.issued.none.fl_str_mv 2020
dc.date.accessioned.none.fl_str_mv 2025-06-23T18:20:21Z
dc.date.available.none.fl_str_mv 2025-06-23T18:20:21Z
dc.date.updated.none.fl_str_mv 2025-06-23T18:20:21Z
dc.type.none.fl_str_mv Article
PublishedVersion
info:eu-repo/semantics/article
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250
url https://ainfo.inia.uy/consulta/busca?b=pc&id=61250&biblioteca=vazio&busca=61250&qFacets=61250
dc.language.iso.none.fl_str_mv en
eng
language_invalid_str_mv en
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.es.fl_str_mv Acceso abierto
eu_rights_str_mv openAccess
rights_invalid_str_mv Acceso abierto
dc.source.none.fl_str_mv reponame:AINFO
instname:Instituto Nacional de Investigación Agropecuaria
instacron:Instituto Nacional de Investigación Agropecuaria
instname_str Instituto Nacional de Investigación Agropecuaria
instacron_str Instituto Nacional de Investigación Agropecuaria
institution Instituto Nacional de Investigación Agropecuaria
reponame_str AINFO
collection AINFO
bitstream.url.fl_str_mv https://redi.anii.org.uy/jspui/bitstream/20.500.12381/4103/1/sword-2025-06-23T15%3a20%3a21.original.xml
bitstream.checksum.fl_str_mv 9ed3751005100bcb6f97039adbf031bc
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv AINFO - Instituto Nacional de Investigación Agropecuaria
repository.mail.fl_str_mv lorrego@inia.org.uy
_version_ 1870824233711435776
score 15,301603

Similares en LA Referencia