Lung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties

Lung cancer is the first leading cause of cancer-related deaths in the world. Aberrant glycosylation in lung tumors leads to the expression of tumor-associated carbohydrate structures, such as the Tn antigen, consisting of N-acetyl-galactosamine (GalNAc) linked to a serine or threonine residue in pr...

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Bibliographic Details
Authors: da Costa, Valeria, Mariño, Karina V., Rodríguez-Zraquia, Santiago A., Festari, María Florencia, Lores, Pablo, Costa, Monique, Landeira, Mercedes, Rabinovich, Gabriel A., van Vliet, Sandra J., Freire, Teresa
Format: article
Status:Published version
Publication Date:2022
Country:Uruguay
Institution:Universidad de la República
Repository:COLIBRI
Language:English
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/54622
Online Access:https://hdl.handle.net/20.500.12008/54622
Access Level:Open access
Keyword:Lung cancer
Tn antigen
Macrophage galactose-type lectin
¨Dendritic cells
O-glycosylation
NEOPLASIAS PULMONARES
MACRÓFAGOS
GALECTINAS
ANTÍGENOS
CÉLULAS DENDRÍTICAS
GLICOSILACIÓN
ACETILGALACTOSAMINA
Description
Summary:Lung cancer is the first leading cause of cancer-related deaths in the world. Aberrant glycosylation in lung tumors leads to the expression of tumor-associated carbohydrate structures, such as the Tn antigen, consisting of N-acetyl-galactosamine (GalNAc) linked to a serine or threonine residue in proteins (α-GalNAc-O-Ser/Thr). The Tn antigen can be recognized by the Macrophage Galactose/GalNAc lectin (MGL), which mediates various immune regulatory and tolerogenic functions, mainly by reprogramming the maturation of function of dendritic cells (DCs). In this work, we generated two different Tn-expressing variants from the Lewis-type lung murine cancer cell line LL/2, which showed different alterations in the O-glycosylation pathways that influenced the interaction with mouse MGL2 and the immunomodulatory properties of DCs. Thus, the identification of the biological programs triggered by Tn+ cancer cells might contribute to an improved understanding of the molecular mechanisms elicited by MGL-dependent immune regulatory circuits.

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