Detection and genotyping of equid herpesvirus 1 in Uruguay

Infection with equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of open reading frame 30 which results in an amino acid...

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Detalles Bibliográficos
Autores: Arbiza, Juan, Castro, E. R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Uruguay
Institución:Universidad de la República
Repositorio:COLIBRI
Idioma:inglés
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/34150
Acceso en línea:https://hdl.handle.net/20.500.12008/34150
Access Level:acceso abierto
Palabra clave:Equid herpesvirus
Genotyping
Lymph node
Molecular detection
Neuropathogenic
Uruguay
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dc.title.none.fl_str_mv Detection and genotyping of equid herpesvirus 1 in Uruguay
title Detection and genotyping of equid herpesvirus 1 in Uruguay
spellingShingle Detection and genotyping of equid herpesvirus 1 in Uruguay
Arbiza, Juan
Equid herpesvirus
Genotyping
Lymph node
Molecular detection
Neuropathogenic
Uruguay
title_short Detection and genotyping of equid herpesvirus 1 in Uruguay
title_full Detection and genotyping of equid herpesvirus 1 in Uruguay
title_fullStr Detection and genotyping of equid herpesvirus 1 in Uruguay
title_full_unstemmed Detection and genotyping of equid herpesvirus 1 in Uruguay
title_sort Detection and genotyping of equid herpesvirus 1 in Uruguay
dc.creator.none.fl_str_mv Arbiza, Juan
Castro, E. R.
author Arbiza, Juan
author_facet Arbiza, Juan
Castro, E. R.
author_role author
author2 Castro, E. R.
author2_role author
dc.contributor.filiacion.none.fl_str_mv Arbiza Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.
Castro E. R.
dc.subject.es.fl_str_mv Equid herpesvirus
Genotyping
Lymph node
Molecular detection
Neuropathogenic
Uruguay
topic Equid herpesvirus
Genotyping
Lymph node
Molecular detection
Neuropathogenic
Uruguay
description Infection with equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of open reading frame 30 which results in an amino acid variation (N752/D752) of the EHV-1 DNA polymerase, is significantly associated with the neuropathogenic potential of naturally occurring strains. In recent years, an increase in the number of cases of equine neurological disease caused by neuropathogenic variants of EHV-1 has been observed in numerous countries. The purpose of this study was to detect the presence of the viral genome of EHV-1 and equid herpesvirus 4 (EHV-4) in the bronchopulmonary lymph nodes of 47 horses from various locations in Uruguay, obtained from a slaughterhouse, and to determine whether the EHV-1 genomes possessed the mutation associated with neuropathogenesis (G2254/D752). The genes encoding glycoprotein H (gH) of EHV-1 and glycoprotein B (gB) of EHV-4 were amplified by a semi-nested polymerase chain reaction. Of the samples analysed, 28% and 6% of lymph nodes contained the genes for gH and gB, respectively. The viral DNA olymerase gene was amplified and sequenced. Twelve of the 13 genomes sequenced presented the nucleotide G2254, while the remaining 1 showed both nucleotides, A2254 and G2254. The results confirm the presence of EHV-1 in Uruguay. Furthermore, there is evidence for the first time of the detection of EHV- 4, and high-frequency detection of the neuropathogenic variant (G2254/D752) of EHV-1 in Uruguay. These findings provide new insights into the epidemiological situation of EHV-1 and EHV-4 in that country.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2022-10-13T12:29:36Z
dc.date.available.none.fl_str_mv 2022-10-13T12:29:36Z
dc.type.es.fl_str_mv Artículo
dc.type.none.fl_str_mv info:eu-repo/semantics/article
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.citation.es.fl_str_mv Arbiza, J. y Castro, E. "Detection and genotyping of equid herpesvirus 1 in Uruguay". OIE Revue Scientifique et Technique. [en línea] 2017, 36 (3): 799-806. 8 h.
dc.identifier.issn.none.fl_str_mv 0253-1933
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12008/34150
dc.identifier.doi.none.fl_str_mv 10.20506/rst36.3.2715
identifier_str_mv Arbiza, J. y Castro, E. "Detection and genotyping of equid herpesvirus 1 in Uruguay". OIE Revue Scientifique et Technique. [en línea] 2017, 36 (3): 799-806. 8 h.
0253-1933
10.20506/rst36.3.2715
url https://hdl.handle.net/20.500.12008/34150
dc.language.iso.none.fl_str_mv en_US
eng
language_invalid_str_mv en_US
language eng
dc.relation.none.fl_str_mv OIE Revue Scientifique et Technique, 2017, 36 (3): 799-806.
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eu_rights_str_mv openAccess
rights_invalid_str_mv Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
dc.format.extent.es.fl_str_mv 8 h.
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dc.publisher.es.fl_str_mv Office International des Epizootes
dc.source.none.fl_str_mv reponame:COLIBRI
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instacron:Universidad de la República
instname_str Universidad de la República
instacron_str Universidad de la República
institution Universidad de la República
reponame_str COLIBRI
collection COLIBRI
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spelling Arbiza Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Castro E. R.2022-10-13T12:29:36Z2022-10-13T12:29:36Z2017Arbiza, J. y Castro, E. "Detection and genotyping of equid herpesvirus 1 in Uruguay". OIE Revue Scientifique et Technique. [en línea] 2017, 36 (3): 799-806. 8 h.0253-1933https://hdl.handle.net/20.500.12008/3415010.20506/rst36.3.2715Infection with equid alphaherpesvirus 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single nucleotide polymorphism (A2254/G2254) in the genome region of open reading frame 30 which results in an amino acid variation (N752/D752) of the EHV-1 DNA polymerase, is significantly associated with the neuropathogenic potential of naturally occurring strains. In recent years, an increase in the number of cases of equine neurological disease caused by neuropathogenic variants of EHV-1 has been observed in numerous countries. The purpose of this study was to detect the presence of the viral genome of EHV-1 and equid herpesvirus 4 (EHV-4) in the bronchopulmonary lymph nodes of 47 horses from various locations in Uruguay, obtained from a slaughterhouse, and to determine whether the EHV-1 genomes possessed the mutation associated with neuropathogenesis (G2254/D752). The genes encoding glycoprotein H (gH) of EHV-1 and glycoprotein B (gB) of EHV-4 were amplified by a semi-nested polymerase chain reaction. Of the samples analysed, 28% and 6% of lymph nodes contained the genes for gH and gB, respectively. The viral DNA olymerase gene was amplified and sequenced. Twelve of the 13 genomes sequenced presented the nucleotide G2254, while the remaining 1 showed both nucleotides, A2254 and G2254. The results confirm the presence of EHV-1 in Uruguay. Furthermore, there is evidence for the first time of the detection of EHV- 4, and high-frequency detection of the neuropathogenic variant (G2254/D752) of EHV-1 in Uruguay. These findings provide new insights into the epidemiological situation of EHV-1 and EHV-4 in that country.Submitted by Farías Verónica (vfarias@fcien.edu.uy) on 2022-10-03T13:35:57Z No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 10.20506rst36.3.2715.pdf: 250840 bytes, checksum: d0047320e6038956121ec41d2ea50a81 (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2022-10-13T12:06:50Z (GMT) No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 10.20506rst36.3.2715.pdf: 250840 bytes, checksum: d0047320e6038956121ec41d2ea50a81 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2022-10-13T12:29:36Z (GMT). No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 10.20506rst36.3.2715.pdf: 250840 bytes, checksum: d0047320e6038956121ec41d2ea50a81 (MD5) Previous issue date: 20178 h.application/pdfen_USengOffice International des EpizootesOIE Revue Scientifique et Technique, 2017, 36 (3): 799-806.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)Equid herpesvirusGenotypingLymph nodeMolecular detectionNeuropathogenicUruguayDetection and genotyping of equid herpesvirus 1 in UruguayArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaArbiza, JuanCastro, E. 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