Soil and rhizosphere bacterial diversity in maize agro-ecosystem.

ABSTRACT.Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diver...

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Detalles Bibliográficos
Autores: FEDERICI, M., BAJSA, N., LAGURARA, P., REVALE, S., MARCONDES, J.A., DALLA RIZZA, M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Uruguay
Institución:Instituto Nacional de Investigación Agropecuaria
Repositorio:AINFO
Idioma:inglés
OAI Identifier:oai:redi.anii.org.uy:20.500.12381/2834
Acceso en línea:https://ainfo.inia.uy/consulta/busca?b=pc&id=57747&biblioteca=vazio&busca=57747&qFacets=57747
Access Level:acceso abierto
Palabra clave:METAGENOMICS
TRANSGENIC MAIZE
PYROSEQUENCING
DGGE
RHIZOSPHERE
SOIL BACTERIA
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spelling 2022-12-16T20:56:26Z2022-12-16T20:56:26Z20172022-12-16T20:56:26Zhttps://ainfo.inia.uy/consulta/busca?b=pc&id=57747&biblioteca=vazio&busca=57747&qFacets=57747ABSTRACT.Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.Copyright © Canadian Center of Science and Educationhttps://hdl.handle.net/20.500.12381/2834enenginfo:eu-repo/semantics/openAccessAcceso abiertoMETAGENOMICSTRANSGENIC MAIZEPYROSEQUENCINGDGGERHIZOSPHERESOIL BACTERIASoil and rhizosphere bacterial diversity in maize agro-ecosystem.ArticlePublishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:AINFOinstname:Instituto Nacional de Investigación Agropecuariainstacron:Instituto Nacional de Investigación AgropecuariaFEDERICI, M.BAJSA, N.LAGURARA, P.REVALE, S.MARCONDES, J.A.DALLA RIZZA, M.SWORDsword-2022-12-16T17:56:26.original.xmlOriginal SWORD entry documentapplication/octet-stream2870https://redi.anii.org.uy/jspui/bitstream/20.500.12381/2834/1/sword-2022-12-16T17%3a56%3a26.original.xmla9cf563e188a1ab5eca0b337d72e74b5MD5120.500.12381/28342026-02-10 15:53:53.073oai:redi.anii.org.uy:20.500.12381/2834Institucionalhttps://ainfo.inia.uy/Organismo científico-tecnológicohttp://inia.uyhttps://redi.anii.org.uy/oai/requestlorrego@inia.org.uyUruguayopendoar:2026-02-10T18:53:53AINFO - Instituto Nacional de Investigación Agropecuariafalse
dc.title.none.fl_str_mv Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
title Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
spellingShingle Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
FEDERICI, M.
METAGENOMICS
TRANSGENIC MAIZE
PYROSEQUENCING
DGGE
RHIZOSPHERE
SOIL BACTERIA
title_short Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
title_full Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
title_fullStr Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
title_full_unstemmed Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
title_sort Soil and rhizosphere bacterial diversity in maize agro-ecosystem.
dc.creator.none.fl_str_mv FEDERICI, M.
BAJSA, N.
LAGURARA, P.
REVALE, S.
MARCONDES, J.A.
DALLA RIZZA, M.
author FEDERICI, M.
author_facet FEDERICI, M.
BAJSA, N.
LAGURARA, P.
REVALE, S.
MARCONDES, J.A.
DALLA RIZZA, M.
author_role author
author2 BAJSA, N.
LAGURARA, P.
REVALE, S.
MARCONDES, J.A.
DALLA RIZZA, M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv METAGENOMICS
TRANSGENIC MAIZE
PYROSEQUENCING
DGGE
RHIZOSPHERE
SOIL BACTERIA
topic METAGENOMICS
TRANSGENIC MAIZE
PYROSEQUENCING
DGGE
RHIZOSPHERE
SOIL BACTERIA
description ABSTRACT.Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.Copyright © Canadian Center of Science and Education
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2022-12-16T20:56:26Z
dc.date.available.none.fl_str_mv 2022-12-16T20:56:26Z
dc.date.updated.none.fl_str_mv 2022-12-16T20:56:26Z
dc.type.none.fl_str_mv Article
PublishedVersion
info:eu-repo/semantics/article
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
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url https://ainfo.inia.uy/consulta/busca?b=pc&id=57747&biblioteca=vazio&busca=57747&qFacets=57747
dc.language.iso.none.fl_str_mv en
eng
language_invalid_str_mv en
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.es.fl_str_mv Acceso abierto
eu_rights_str_mv openAccess
rights_invalid_str_mv Acceso abierto
dc.source.none.fl_str_mv reponame:AINFO
instname:Instituto Nacional de Investigación Agropecuaria
instacron:Instituto Nacional de Investigación Agropecuaria
instname_str Instituto Nacional de Investigación Agropecuaria
instacron_str Instituto Nacional de Investigación Agropecuaria
institution Instituto Nacional de Investigación Agropecuaria
reponame_str AINFO
collection AINFO
bitstream.url.fl_str_mv https://redi.anii.org.uy/jspui/bitstream/20.500.12381/2834/1/sword-2022-12-16T17%3a56%3a26.original.xml
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repository.mail.fl_str_mv lorrego@inia.org.uy
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