Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases

Cells are constantly adapting to maintain their identity in response to the surrounding media's temporal and spatial heterogeneity. The plasma membrane, which participates in the transduction of external signals, plays a crucial role in this adaptation. Studies suggest that nano and micrometer...

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Autores: Aguilar, Joao, Malacrida, Leonel, Gunther, Germán, Torrado, Belén, Urbano, Bruno F., Torres, Viviana, Sánchez, Susana A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Uruguay
Institución:Universidad de la República
Repositorio:COLIBRI
Idioma:inglés
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/52077
Acceso en línea:https://hdl.handle.net/20.500.12008/52077
Access Level:acceso abierto
Palabra clave:Membrane heterogeneity
Spectral phasor analysis
LAURDAN
Membrane fluidity
Collagen matrices
Extracellular stiffness
ESTRUCTURAS DE LA MEMBRANA CELULAR
METABOLISMO
COLÁGENO
LAURATOS
QUÍMICA
FLUIDEZ DE LA MEMBRANA
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dc.title.none.fl_str_mv Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
title Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
spellingShingle Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
Aguilar, Joao
Membrane heterogeneity
Spectral phasor analysis
LAURDAN
Membrane fluidity
Collagen matrices
Extracellular stiffness
ESTRUCTURAS DE LA MEMBRANA CELULAR
METABOLISMO
COLÁGENO
LAURATOS
QUÍMICA
FLUIDEZ DE LA MEMBRANA
title_short Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
title_full Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
title_fullStr Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
title_full_unstemmed Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
title_sort Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases
dc.creator.none.fl_str_mv Aguilar, Joao
Malacrida, Leonel
Gunther, Germán
Torrado, Belén
Urbano, Bruno F.
Torres, Viviana
Sánchez, Susana A.
author Aguilar, Joao
author_facet Aguilar, Joao
Malacrida, Leonel
Gunther, Germán
Torrado, Belén
Urbano, Bruno F.
Torres, Viviana
Sánchez, Susana A.
author_role author
author2 Malacrida, Leonel
Gunther, Germán
Torrado, Belén
Urbano, Bruno F.
Torres, Viviana
Sánchez, Susana A.
author2_role author
author
author
author
author
author
dc.contributor.filiacion.none.fl_str_mv Aguilar Joao, Universidad de Concepción (Chile). Facultad de Ciencias Químicas
Malacrida Leonel, Universidad de la República (Uruguay). Facultad de Medicina
Gunther Germán, Universidad de Chile (Chile). Departamento de Química Orgánica y Fisicoquímica
Torrado Belén, University of California at Irvine (E.E.U.U.). Biomedical Engineering Department
Urbano Bruno F., Universidad de Concepción (Chile). Facultad de Ciencias Químicas
Torres Viviana, Universidad de Concepción (Chile). Facultad de Ciencias Biológicas
Sánchez Susana A., Universidad de Concepción (Chile). Facultad de Ciencias Químicas
dc.subject.es.fl_str_mv Membrane heterogeneity
Spectral phasor analysis
LAURDAN
Membrane fluidity
Collagen matrices
Extracellular stiffness
topic Membrane heterogeneity
Spectral phasor analysis
LAURDAN
Membrane fluidity
Collagen matrices
Extracellular stiffness
ESTRUCTURAS DE LA MEMBRANA CELULAR
METABOLISMO
COLÁGENO
LAURATOS
QUÍMICA
FLUIDEZ DE LA MEMBRANA
dc.subject.other.es.fl_str_mv ESTRUCTURAS DE LA MEMBRANA CELULAR
METABOLISMO
COLÁGENO
LAURATOS
QUÍMICA
FLUIDEZ DE LA MEMBRANA
description Cells are constantly adapting to maintain their identity in response to the surrounding media's temporal and spatial heterogeneity. The plasma membrane, which participates in the transduction of external signals, plays a crucial role in this adaptation. Studies suggest that nano and micrometer areas with different fluidities at the plasma membrane change their distribution in response to external mechanical signals. However, investigations linking fluidity domains with mechanical stimuli, specifically matrix stiffness, are still in progress. This report tests the hypothesis that the stiffness of the extracellular matrix can modify the equilibrium of areas with different order in the plasma membrane, resulting in changes in overall membrane fluidity distribution. We studied the effect of matrix stiffness on the distribution of membrane lipid domains in NIH-3 T3 cells immersed in matrices of varying concentrations of collagen type I, for 24 or 72 h. The stiffness and viscoelastic properties of the collagen matrices were characterized by rheometry, fiber sizes were measured by Scanning Electron Microscopy (SEM) and the volume occupied by the fibers by second harmonic generation imaging (SHG). Membrane fluidity was measured using the fluorescent dye LAURDAN and spectral phasor analysis. The results demonstrate that an increase in collagen stiffness alters the distribution of membrane fluidity, leading to an increasing amount of the LAURDAN fraction with a high degree of packing. These findings suggest that changes in the equilibrium of fluidity domains could represent a versatile and refined component of the signal transduction mechanism for cells to respond to the highly heterogeneous matrix structural composition. Overall, this study sheds light on the importance of the plasma membrane's role in adapting to the extracellular matrix's mechanical cues.
publishDate 2023
dc.date.issued.none.fl_str_mv 2023
dc.date.accessioned.none.fl_str_mv 2025-10-15T12:52:17Z
dc.date.available.none.fl_str_mv 2025-10-15T12:52:17Z
dc.type.es.fl_str_mv Artículo
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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format article
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dc.identifier.citation.es.fl_str_mv Aguilar J, Malacrida L, Gunther G y otros. Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases. Biochimica et Biophysica Acta (BBA) - Biomembranes [en línea]. 2023;1865(7). 10 p.
dc.identifier.issn.none.fl_str_mv 1879-2642
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12008/52077
dc.identifier.doi.none.fl_str_mv 10.1016/j.bbamem.2023.184176
identifier_str_mv Aguilar J, Malacrida L, Gunther G y otros. Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases. Biochimica et Biophysica Acta (BBA) - Biomembranes [en línea]. 2023;1865(7). 10 p.
1879-2642
10.1016/j.bbamem.2023.184176
url https://hdl.handle.net/20.500.12008/52077
dc.language.iso.none.fl_str_mv en
eng
language_invalid_str_mv en
language eng
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta (BBA) - Biomembranes. 2023;1865(7)
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.license.none.fl_str_mv Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
dc.format.extent.es.fl_str_mv 10 p.
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dc.publisher.es.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:COLIBRI
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instacron_str Universidad de la República
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spelling Aguilar Joao, Universidad de Concepción (Chile). Facultad de Ciencias QuímicasMalacrida Leonel, Universidad de la República (Uruguay). Facultad de MedicinaGunther Germán, Universidad de Chile (Chile). Departamento de Química Orgánica y FisicoquímicaTorrado Belén, University of California at Irvine (E.E.U.U.). Biomedical Engineering DepartmentUrbano Bruno F., Universidad de Concepción (Chile). Facultad de Ciencias QuímicasTorres Viviana, Universidad de Concepción (Chile). Facultad de Ciencias BiológicasSánchez Susana A., Universidad de Concepción (Chile). Facultad de Ciencias Químicas2025-10-15T12:52:17Z2025-10-15T12:52:17Z2023Aguilar J, Malacrida L, Gunther G y otros. Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases. Biochimica et Biophysica Acta (BBA) - Biomembranes [en línea]. 2023;1865(7). 10 p.1879-2642https://hdl.handle.net/20.500.12008/5207710.1016/j.bbamem.2023.184176Cells are constantly adapting to maintain their identity in response to the surrounding media's temporal and spatial heterogeneity. The plasma membrane, which participates in the transduction of external signals, plays a crucial role in this adaptation. Studies suggest that nano and micrometer areas with different fluidities at the plasma membrane change their distribution in response to external mechanical signals. However, investigations linking fluidity domains with mechanical stimuli, specifically matrix stiffness, are still in progress. This report tests the hypothesis that the stiffness of the extracellular matrix can modify the equilibrium of areas with different order in the plasma membrane, resulting in changes in overall membrane fluidity distribution. We studied the effect of matrix stiffness on the distribution of membrane lipid domains in NIH-3 T3 cells immersed in matrices of varying concentrations of collagen type I, for 24 or 72 h. The stiffness and viscoelastic properties of the collagen matrices were characterized by rheometry, fiber sizes were measured by Scanning Electron Microscopy (SEM) and the volume occupied by the fibers by second harmonic generation imaging (SHG). Membrane fluidity was measured using the fluorescent dye LAURDAN and spectral phasor analysis. The results demonstrate that an increase in collagen stiffness alters the distribution of membrane fluidity, leading to an increasing amount of the LAURDAN fraction with a high degree of packing. These findings suggest that changes in the equilibrium of fluidity domains could represent a versatile and refined component of the signal transduction mechanism for cells to respond to the highly heterogeneous matrix structural composition. Overall, this study sheds light on the importance of the plasma membrane's role in adapting to the extracellular matrix's mechanical cues.Submitted by Almiñana María Cecilia (marialminana@gmail.com) on 2025-09-19T16:20:10Z No. of bitstreams: 2 license_rdf: 26539 bytes, checksum: 3b50ae24bd8bd076d49a70878a8a2d2c (MD5) Cells immersed in collagen matrices.pdf: 7756281 bytes, checksum: 25b2d9abfc5563cbc3c58c3bf9440eac (MD5)Approved for entry into archive by Almiñana María Cecilia (marialminana@gmail.com) on 2025-10-08T19:23:12Z (GMT) No. of bitstreams: 2 license_rdf: 26539 bytes, checksum: 3b50ae24bd8bd076d49a70878a8a2d2c (MD5) Cells immersed in collagen matrices.pdf: 7756281 bytes, checksum: 25b2d9abfc5563cbc3c58c3bf9440eac (MD5)Rejected by Luna Fabiana (fabiana.luna@seciu.edu.uy), reason: Hola Cecilia te mando este otro artículo, porque la versión publicada tiene asociada tarifas, según figura en Sherpa Romeo, la única versión que se puede subir son los pre o post print. Quizás les convenga conseguir el post o preprint para poder depositarlo en el repositorio. https://openpolicyfinder.jisc.ac.uk/id/publication/12429 Saludos Fabiana on 2025-10-10T15:27:02Z (GMT)Submitted by Almiñana María Cecilia (marialminana@gmail.com) on 2025-10-14T16:44:59Z No. of bitstreams: 2 license_rdf: 26539 bytes, checksum: 3b50ae24bd8bd076d49a70878a8a2d2c (MD5) Cells immersed in collagen matrices.pdf: 7756281 bytes, checksum: 25b2d9abfc5563cbc3c58c3bf9440eac (MD5)Approved for entry into archive by Almiñana María Cecilia (marialminana@gmail.com) on 2025-10-14T16:45:24Z (GMT) No. of bitstreams: 2 license_rdf: 26539 bytes, checksum: 3b50ae24bd8bd076d49a70878a8a2d2c (MD5) Cells immersed in collagen matrices.pdf: 7756281 bytes, checksum: 25b2d9abfc5563cbc3c58c3bf9440eac (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2025-10-15T12:52:17Z (GMT). No. of bitstreams: 2 license_rdf: 26539 bytes, checksum: 3b50ae24bd8bd076d49a70878a8a2d2c (MD5) Cells immersed in collagen matrices.pdf: 7756281 bytes, checksum: 25b2d9abfc5563cbc3c58c3bf9440eac (MD5) Previous issue date: 202310 p.application/pdfenengElsevierBiochimica et Biophysica Acta (BBA) - Biomembranes. 2023;1865(7)Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)Membrane heterogeneitySpectral phasor analysisLAURDANMembrane fluidityCollagen matricesExtracellular stiffnessESTRUCTURAS DE LA MEMBRANA CELULARMETABOLISMOCOLÁGENOLAURATOSQUÍMICAFLUIDEZ DE LA MEMBRANACells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increasesArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaAguilar, JoaoMalacrida, LeonelGunther, GermánTorrado, BelénUrbano, Bruno F.Torres, VivianaSánchez, Susana A.LICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/52077/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; 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públicahttps://udelar.edu.uy/https://www.colibri.udelar.edu.uy/oai/requestkarina.camps@seciu.edu.uyUruguayopendoar:47712025-10-15T12:52:17COLIBRI - Universidad de la Repúblicafalse
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