Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers

Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as F...

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Autores: McNulty, S., Tort, José F., Rinaldi, Gabriel, Fischer, K., Rosa, B. A., Smircich, Pablo, Fontenla Martínez, Santiago, Choi, Y., Tyagi, R., Hallsworth-Pepin, K., Mann, H. M., Kammili, L., Latham, P. S., Dell'Oca Runco, Nicolás, Domínguez, María Fernanda, Carmona García, Carlos, Fischer, P. U., Brindley, P. J., Mitreva, M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Uruguay
Institución:Universidad de la República
Repositorio:COLIBRI
Idioma:inglés
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/22412
Acceso en línea:https://hdl.handle.net/20.500.12008/22412
Access Level:acceso abierto
Palabra clave:Trematodos transmitidos por alimentos
FBT
Fasciola hepática
Genética
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dc.title.none.fl_str_mv Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
title Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
spellingShingle Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
McNulty, S.
Trematodos transmitidos por alimentos
FBT
Fasciola hepática
Genética
title_short Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
title_full Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
title_fullStr Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
title_full_unstemmed Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
title_sort Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers
dc.creator.none.fl_str_mv McNulty, S.
Tort, José F.
Rinaldi, Gabriel
Fischer, K.
Rosa, B. A.
Smircich, Pablo
Fontenla Martínez, Santiago
Choi, Y.
Tyagi, R.
Hallsworth-Pepin, K.
Mann, H. M.
Kammili, L.
Latham, P. S.
Dell'Oca Runco, Nicolás
Domínguez, María Fernanda
Carmona García, Carlos
Fischer, P. U.
Brindley, P. J.
Mitreva, M.
author McNulty, S.
author_facet McNulty, S.
Tort, José F.
Rinaldi, Gabriel
Fischer, K.
Rosa, B. A.
Smircich, Pablo
Fontenla Martínez, Santiago
Choi, Y.
Tyagi, R.
Hallsworth-Pepin, K.
Mann, H. M.
Kammili, L.
Latham, P. S.
Dell'Oca Runco, Nicolás
Domínguez, María Fernanda
Carmona García, Carlos
Fischer, P. U.
Brindley, P. J.
Mitreva, M.
author_role author
author2 Tort, José F.
Rinaldi, Gabriel
Fischer, K.
Rosa, B. A.
Smircich, Pablo
Fontenla Martínez, Santiago
Choi, Y.
Tyagi, R.
Hallsworth-Pepin, K.
Mann, H. M.
Kammili, L.
Latham, P. S.
Dell'Oca Runco, Nicolás
Domínguez, María Fernanda
Carmona García, Carlos
Fischer, P. U.
Brindley, P. J.
Mitreva, M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.filiacion.none.fl_str_mv McNulty S.
Tort Jose F., Universidad de la República (Uruguay). Facultad de Medicina
Rinaldi G.
Fischer K.
Rosa B. A.
Smircich Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología
Fontenla Santiago, Universidad de la República (Uruguay). Facultad de Medicina
Choi Y.
Tyagi R.
Hallsworth-Pepin K.
Mann H.M.
Kammili L.
Latham P. S.
Dell'Oca Runco Nicolás, Universidad de la República (Uruguay). Facultad de Medicina
Dominguez Fernanda, Universidad de la República (Uruguay). Facultad de Medicina
Carmona Carlos, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología
Fischer P. U.
Brindley P. J.
Mitreva M
dc.subject.es.fl_str_mv Trematodos transmitidos por alimentos
FBT
Fasciola hepática
Genética
topic Trematodos transmitidos por alimentos
FBT
Fasciola hepática
Genética
description Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered transkingdom interaction with nFh and the impact of both species on disease in ruminants and humans.
publishDate 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2019-11-18T16:14:04Z
dc.date.available.none.fl_str_mv 2019-11-18T16:14:04Z
dc.type.es.fl_str_mv Artículo
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dc.identifier.citation.es.fl_str_mv MCNULTY, S., y otros. "Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers" PLoS Genetics [en línea]. 2017, 13(1): e1006537. doi: 10.1371/journal.pgen.1006537
dc.identifier.issn.none.fl_str_mv 1553-7404
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12008/22412
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pgen.1006537
identifier_str_mv MCNULTY, S., y otros. "Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers" PLoS Genetics [en línea]. 2017, 13(1): e1006537. doi: 10.1371/journal.pgen.1006537
1553-7404
10.1371/journal.pgen.1006537
url https://hdl.handle.net/20.500.12008/22412
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dc.relation.none.fl_str_mv PLoS Genetics, 2017, 13(1): e1006537
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spelling McNulty S.Tort Jose F., Universidad de la República (Uruguay). Facultad de MedicinaRinaldi G.Fischer K.Rosa B. A.Smircich Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaFontenla Santiago, Universidad de la República (Uruguay). Facultad de MedicinaChoi Y.Tyagi R.Hallsworth-Pepin K.Mann H.M.Kammili L.Latham P. S.Dell'Oca Runco Nicolás, Universidad de la República (Uruguay). Facultad de MedicinaDominguez Fernanda, Universidad de la República (Uruguay). Facultad de MedicinaCarmona Carlos, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaFischer P. U.Brindley P. J.Mitreva M2019-11-18T16:14:04Z2019-11-18T16:14:04Z2017MCNULTY, S., y otros. "Genomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu Fevers" PLoS Genetics [en línea]. 2017, 13(1): e1006537. doi: 10.1371/journal.pgen.10065371553-7404https://hdl.handle.net/20.500.12008/2241210.1371/journal.pgen.1006537Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. hepatica Oregon and related food-borne trematodes are metabolically less constrained than schistosomes and cestodes, taking advantage of the richer millieux offered by the hepatobiliary organs. Protease families differentially expanded between diverse trematodes may facilitate migration and survival within the heterogeneous environments and niches within the mammalian host. Surprisingly, the sequencing of Oregon and Uruguay F. hepatica isolates led to the first discovery of an endobacteria in this species. Two contigs from the F. hepatica Oregon assembly were joined to complete the 859,205 bp genome of a novel Neorickettsia endobacterium (nFh) closely related to the etiological agents of human Sennetsu and Potomac horse fevers. Immunohistochemical studies targeting a Neorickettsia surface protein found nFh in specific organs and tissues of the adult trematode including the female reproductive tract, eggs, the Mehlis' gland, seminal vesicle, and oral suckers, suggesting putative routes for fluke-to-fluke and fluke-to-host transmission. The genomes of F. hepatica and nFh will serve as a resource for further exploration of the biology of F. hepatica, and specifically its newly discovered transkingdom interaction with nFh and the impact of both species on disease in ruminants and humans.Submitted by Faget Cecilia (lfaget@fcien.edu.uy) on 2019-11-18T13:35:47Z No. of bitstreams: 2 license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5) 101371journalpgen1006537.pdf: 3316033 bytes, checksum: 441c8ada4d0e800fa1fd3ea236f9ca8a (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2019-11-18T15:26:27Z (GMT) No. of bitstreams: 2 license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5) 101371journalpgen1006537.pdf: 3316033 bytes, checksum: 441c8ada4d0e800fa1fd3ea236f9ca8a (MD5)Made available in DSpace on 2019-11-18T16:14:04Z (GMT). No. of bitstreams: 2 license_rdf: 19874 bytes, checksum: 38cb62ef53e6f513db2fb7e337df6485 (MD5) 101371journalpgen1006537.pdf: 3316033 bytes, checksum: 441c8ada4d0e800fa1fd3ea236f9ca8a (MD5) Previous issue date: 201725 h.application/pdfenengPLoSPLoS Genetics, 2017, 13(1): e1006537Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)Trematodos transmitidos por alimentosFBTFasciola hepáticaGenéticaGenomes of Fasciola hepatica from the Americas reveal colonization with Neorickettsia Endobacteria related to the agents of Potomac Horse and Human Sennetsu FeversArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaMcNulty, S.Tort, José F.Rinaldi, GabrielFischer, K.Rosa, B. A.Smircich, PabloFontenla Martínez, SantiagoChoi, Y.Tyagi, R.Hallsworth-Pepin, K.Mann, H. M.Kammili, L.Latham, P. S.Dell'Oca Runco, NicolásDomínguez, María FernandaCarmona García, CarlosFischer, P. U.Brindley, P. 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públicahttps://udelar.edu.uy/https://www.colibri.udelar.edu.uy/oai/requestkarina.camps@seciu.edu.uyUruguayopendoar:47712026-05-22T15:18:20COLIBRI - Universidad de la Repúblicafalse
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