A deletion in SARS-CoV- 2 ORF7 identified in COVID-19 outbreak in Uruguay

The analysis of genetic diversity in SARS-CoV-2 is the focus of several studies, providing insights into how the virus emerged and evolves. Most common changes in SARS-CoV-2 are single or point nucleotide substitutions; meanwhile, insertions and deletions (indels) have been identified as a less freq...

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Detalhes bibliográficos
Autores: Panzera Crespo, Yanina, Ramos, Natalia, Frabasile Giurato, Sandra Alicia, Calleros Basilio, Lucía, Marandino, Ana, Tomás Custodio, Gonzalo Martín, Techera, Claudia, Grecco Patiño, Sofía, Fuques Villalba, Eddie, Goñi Mazzitelli, Natalia, Ramas, Vivivana, Coppola, Leticia, Chiparelli, Héctor, Sorhouet, Cecilia, Mogdasy, Cristina, Arbiza, Juan, Delfraro Vázquez, Adriana Beatriz, Pérez Crossa, Ruben Gustavo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Uruguay
Recursos:Universidad de la República
Repositorio:COLIBRI
Idioma:inglés
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/34911
Acesso em linha:https://hdl.handle.net/20.500.12008/34911
Access Level:acceso abierto
Palavra-chave:SARS-CoV- 2
ORF7a
Descrição
Resumo:The analysis of genetic diversity in SARS-CoV-2 is the focus of several studies, providing insights into how the virus emerged and evolves. Most common changes in SARS-CoV-2 are single or point nucleotide substitutions; meanwhile, insertions and deletions (indels) have been identified as a less frequent source of viral genetic variability. Here, we report the emergence of a 12-nucleotide deletion in ORF7a, resulting in a 4-amino acid in-frame deletion. The Δ12 variant was identified in viruses from patients of a single outbreak and represents the first report of this deletion in South American isolates. Phylogenetic analysis revealed that Δ12 strains belong to the lineage B.1.1 and clustered separated from the remaining Uruguayan strains. The ∆12 variant was detected in 14 patients of this outbreak by NGS sequencing and/or two rapid and economic methodologies: Sanger amplicon sequencing and capillary electrophoresis. The presence of strong molecular markers as the deletion described here are useful for tracking outbreaks and reveal a significant aspect of the SARS-CoV-2 evolution on the robustness of the virus to keep its functionality regardless loss of genetic material.

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