Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system

The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins wer...

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Autores: García-Roche Saracco, María Mercedes, Talmón Travers, Daniel, Cañibe, Guillermo, Astessiano, Ana Laura, Mendoza, Alejandro, Quijano, Daniel, Cassina, Adriana, Carriquiry Fossemale, Mariana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Uruguay
Institución:Universidad de la República
Repositorio:COLIBRI
Idioma:inglés
OAI Identifier:oai:colibri.udelar.edu.uy:20.500.12008/43074
Acceso en línea:https://hdl.handle.net/20.500.12008/43074
https://doi.org/10.3168/jds.2021-21358
Access Level:acceso abierto
Palabra clave:Intermediary metabolism
Gluconeogenesis
Dairy cows
Grazing
Holstein-Friesian
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dc.title.none.fl_str_mv Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
title Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
spellingShingle Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
García-Roche Saracco, María Mercedes
Intermediary metabolism
Gluconeogenesis
Dairy cows
Grazing
Holstein-Friesian
title_short Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
title_full Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
title_fullStr Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
title_full_unstemmed Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
title_sort Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
dc.creator.none.fl_str_mv García-Roche Saracco, María Mercedes
Talmón Travers, Daniel
Cañibe, Guillermo
Astessiano, Ana Laura
Mendoza, Alejandro
Quijano, Daniel
Cassina, Adriana
Carriquiry Fossemale, Mariana
author García-Roche Saracco, María Mercedes
author_facet García-Roche Saracco, María Mercedes
Talmón Travers, Daniel
Cañibe, Guillermo
Astessiano, Ana Laura
Mendoza, Alejandro
Quijano, Daniel
Cassina, Adriana
Carriquiry Fossemale, Mariana
author_role author
author2 Talmón Travers, Daniel
Cañibe, Guillermo
Astessiano, Ana Laura
Mendoza, Alejandro
Quijano, Daniel
Cassina, Adriana
Carriquiry Fossemale, Mariana
author2_role author
author
author
author
author
author
author
dc.contributor.filiacion.none.fl_str_mv García-Roche Saracco María Mercedes
Talmón Travers Daniel
Cañibe Guillermo
Astessiano Ana Laura
Mendoza Alejandro
Quijano Daniel
Cassina Adriana
Carriquiry Fossemale Mariana
dc.subject.es.fl_str_mv Intermediary metabolism
Gluconeogenesis
Dairy cows
Grazing
Holstein-Friesian
topic Intermediary metabolism
Gluconeogenesis
Dairy cows
Grazing
Holstein-Friesian
description The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures.
publishDate 2022
dc.date.issued.none.fl_str_mv 2022
dc.date.accessioned.none.fl_str_mv 2024-03-14T12:30:59Z
dc.date.available.none.fl_str_mv 2024-03-14T12:30:59Z
dc.type.es.fl_str_mv Artículo
dc.type.none.fl_str_mv info:eu-repo/semantics/article
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.citation.es.fl_str_mv García-Roche Saracco, M, Talmón Travers, D, Cañibe, G. "Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system". Journal of Dairy Science. [en línea] 2022, 105(7): 5723-5737
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12008/43074
dc.identifier.doi.none.fl_str_mv https://doi.org/10.3168/jds.2021-21358
identifier_str_mv García-Roche Saracco, M, Talmón Travers, D, Cañibe, G. "Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system". Journal of Dairy Science. [en línea] 2022, 105(7): 5723-5737
url https://hdl.handle.net/20.500.12008/43074
https://doi.org/10.3168/jds.2021-21358
dc.language.iso.none.fl_str_mv en
eng
language_invalid_str_mv en
language eng
dc.relation.none.fl_str_mv Journal of Dairy Science, 105(7): 5723-5737
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.license.none.fl_str_mv Licencia Creative Commons Atribución (CC - By 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv Licencia Creative Commons Atribución (CC - By 4.0)
dc.format.mimetype.es.fl_str_mv application/pdf
dc.publisher.es.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:COLIBRI
instname:Universidad de la República
instacron:Universidad de la República
instname_str Universidad de la República
instacron_str Universidad de la República
institution Universidad de la República
reponame_str COLIBRI
collection COLIBRI
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spelling García-Roche Saracco María MercedesTalmón Travers DanielCañibe GuillermoAstessiano Ana LauraMendoza AlejandroQuijano DanielCassina AdrianaCarriquiry Fossemale Mariana2024-03-14T12:30:59Z2024-03-14T12:30:59Z2022García-Roche Saracco, M, Talmón Travers, D, Cañibe, G. "Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system". Journal of Dairy Science. [en línea] 2022, 105(7): 5723-5737https://hdl.handle.net/20.500.12008/43074https://doi.org/10.3168/jds.2021-21358The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures.Submitted by Muniz Andrea (rosmeri8@hotmail.com) on 2024-03-14T12:14:21Z No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) ResumenGarcia Roche et al. 2022-JDS.pdf: 699659 bytes, checksum: 0cbf80e0f38d4eec5817bc9da3e500e8 (MD5)Approved for entry into archive by Muniz Andrea (rosmeri8@hotmail.com) on 2024-03-14T12:25:48Z (GMT) No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) ResumenGarcia Roche et al. 2022-JDS.pdf: 699659 bytes, checksum: 0cbf80e0f38d4eec5817bc9da3e500e8 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2024-03-14T12:30:59Z (GMT). No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) ResumenGarcia Roche et al. 2022-JDS.pdf: 699659 bytes, checksum: 0cbf80e0f38d4eec5817bc9da3e500e8 (MD5) Previous issue date: 2022application/pdfenengSpringerJournal of Dairy Science, 105(7): 5723-5737Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)Intermediary metabolismGluconeogenesisDairy cowsGrazingHolstein-FriesianDifferential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based systemArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaGarcía-Roche Saracco, María MercedesTalmón Travers, DanielCañibe, GuillermoAstessiano, Ana LauraMendoza, AlejandroQuijano, DanielCassina, AdrianaCarriquiry Fossemale, MarianaLICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/43074/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; 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públicahttps://udelar.edu.uy/https://www.colibri.udelar.edu.uy/oai/requestkarina.camps@seciu.edu.uyUruguayopendoar:47712025-02-14T19:03:59COLIBRI - Universidad de la Repúblicafalse
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