Nitrocellulose Membrane-Based Enzyme-Linked Immunoassay for Dengue Serotype-1 IgM Detection
OBJETIVES: To evaluate the sensitivity and specifity of a nitrocellulose membrane-based immunoassay for dengue IgM, with respect to capture enzyme immunoassay, for the diagnosis of dengue virus infection. METHODS: 101 serum samples were processed and divided into 2 groups: 53 from dengue serotype 1...
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| Format: | article |
| Status: | Published version |
| Publication Date: | 1999 |
| Country: | Perú |
| Institution: | Universidad Nacional Mayor de San Marcos |
| Repository: | Revistas - Universidad Nacional Mayor de San Marcos |
| Language: | Spanish |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/4475 |
| Online Access: | https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/4475 |
| Access Level: | Open access |
| Keyword: | Dengue Collodion Immunoassay IgM Colodion Inmunoensayo |
| Summary: | OBJETIVES: To evaluate the sensitivity and specifity of a nitrocellulose membrane-based immunoassay for dengue IgM, with respect to capture enzyme immunoassay, for the diagnosis of dengue virus infection. METHODS: 101 serum samples were processed and divided into 2 groups: 53 from dengue serotype 1 (DEN1) infected patients, and 48 from healthy subjects. Both groups were tested with a nitrocellulose membrane-based IgM capture enzyme immunoassay (NMB-EIA) and also with an ELISA as referential pattern. RESULTS: NMB-EIA testing detected IgM anti-DEN1 in 94,34% of samples from infected patients, and in 14,58% of control samples, whereas ELISA fails to report false positive or false negative results. CONCLUSION: NMB-EIA appears to be a good alternative for dengue infection diagnosis. |
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