Effectiveness of an autovaccine in a case of canine papillomavirus in a Great Dane dog in Costa Rica
The aim of this study was to determine the effectiveness of an autovaccine for papilloma caused by canine papillomavirus (CPV) in the facial region in a three-month-old Great Dane dog. A group of papillomas were collected from the patient’s face and analyzed using molecular techniques (amplification...
| Autores: | , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Perú |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Idioma: | español |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/20187 |
| Acceso en línea: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20187 |
| Access Level: | acceso abierto |
| Palabra clave: | genotipo secuenciación papilomavirus patología autovacuna PCR hiperqueratosis genotype sequencing papillomavirus pathology autovaccine hyperkeratosis |
| Sumario: | The aim of this study was to determine the effectiveness of an autovaccine for papilloma caused by canine papillomavirus (CPV) in the facial region in a three-month-old Great Dane dog. A group of papillomas were collected from the patient’s face and analyzed using molecular techniques (amplification of a segment of the gene that codes for the L1 protein by PCR and sequencing). For sequence analysis, the BLAST algorithm was used. The histological study was performed by hematoxylin-eosin staining of the tissue and analysis under a 20X light microscope. An autovaccine was developed from the excised papillomas, which was administered intradermally on four occasions at seven-day intervals. The presence of genotype 1 CPV was established in the papilloma analyzed. Histopathology established the formation of exophytic epidermal papillae supported by a fibrovascular chorion with hyperkeratosis (orthokeratotic) and hyperplasia of the stratum spinosum and with keratinocytes (koilocytes). The papillomas disappeared after the third application of the autovaccine. This is the first CPV-1 report in Costa Rica. The autovaccine proved to be an effective treatment in the management of facial papillomatosis in the patient. |
|---|