Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin

In order to isolate halotolerant bacteria with lipolytic activity from the Pilluana Salterns in San Martin, saline soil samples were collected and cultured in 5% salt water agar containing 0,5% yeast extract. It was pre-selected 55 bacteria with different morphological characteristics, these bacteri...

Descripción completa

Detalles Bibliográficos
Autores: Flores-Fernández, Mónica L., Zavaleta, Amparo I., Chávez-Hidalgo, Elizabeth L.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Perú
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Idioma:español
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/3232
Acceso en línea:https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/3232
Access Level:acceso abierto
Palabra clave:Bacterias halotolerantes
bacterias lipolíticas
suelos salinos
Salinas de Pilluana.
Halotolerant bacteria
lipolytic bacteria
salty soil
Pilluana Salterns.
id PE_d0fd64bd1a86e2237b94dea2334010bd
oai_identifier_str oai:revistasinvestigacion.unmsm.edu.pe:article/3232
network_acronym_str PE
network_name_str Perú
repository_id_str
spelling Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San MartinBacterias halotolerantes con actividad lipolítica aisladas de las Salinas de Pilluana - San MartínFlores-Fernández, Mónica L.Zavaleta, Amparo I.Chávez-Hidalgo, Elizabeth L.Bacterias halotolerantesbacterias lipolíticassuelos salinosSalinas de Pilluana.Halotolerant bacterialipolytic bacteriasalty soilPilluana Salterns.In order to isolate halotolerant bacteria with lipolytic activity from the Pilluana Salterns in San Martin, saline soil samples were collected and cultured in 5% salt water agar containing 0,5% yeast extract. It was pre-selected 55 bacteria with different morphological characteristics, these bacteria were grown simultaneously in 5% salt water agar containing 1% tributyrin, Tween 80 and olive oil. It was selected 14 bacteria based on the ability to hydrolyze specific substrates and shown high salt tolerance rate between 0 to 25%. These bacteria grew optimally at pH 7 and 37 °C, six were Gram positive and Gram negative eight, 12 bacteria hydrolyzed tributyrin, Tween 80 and olive oil, six produced amylases and proteases. The 14 halotolerant bacteria with lipolytic activity isolated from the Pilluana salterns are promising lipase production sources and oily waste bio-treatment.Con el objetivo de aislar bacterias halotolerantes con actividad lipolítica de las Salinas de Pilluana - San Martín, se recolectaron muestras de suelos salinos y se sembraron en agar agua de sales al 5% conteniendo extracto de levadura 0,5%. Se preseleccionaron 55 bacterias con características morfológicas diferentes, sembrándose simultáneamente en agar agua de sales al 5% conteniendo tributirina, Tween 80 y aceite de oliva al 1%. En base a la capacidad de hidrolizar los sustratos específicos y presentar tolerancia salina variable desde 0 hasta 25%, se seleccionaron 14 bacterias que crecieron óptimamente a 37 °C y pH 7, seis fueron bacilos Gram positivos y ocho Gram negativos; 12 de las bacterias hidrolizaron tributirina, Tween 80 y aceite de oliva; seis produjeron amilasas y proteasas. Las 14 bacterias halotolerantes con actividad lipolítica aisladas de las Salinas de Pilluana constituyen fuentes promisorias para la producción de lipasas y biotratamiento de residuos oleosos.Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica2010-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/323210.15381/ci.v13i2.3232Ciencia e Investigación; Vol. 13 Núm. 2 (2010); 88-92Ciencia e Investigación; Vol. 13 No. 2 (2010); 88-921609-90441561-0861reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/3232/2700Derechos de autor 2010 Mónica L. Flores-Fernández, Amparo I. Zavaleta, Elizabeth L. Chávez-Hidalgohttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:revistasinvestigacion.unmsm.edu.pe:article/32322020-04-27T09:47:53Z
dc.title.none.fl_str_mv Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
Bacterias halotolerantes con actividad lipolítica aisladas de las Salinas de Pilluana - San Martín
title Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
spellingShingle Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
Flores-Fernández, Mónica L.
Bacterias halotolerantes
bacterias lipolíticas
suelos salinos
Salinas de Pilluana.
Halotolerant bacteria
lipolytic bacteria
salty soil
Pilluana Salterns.
title_short Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
title_full Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
title_fullStr Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
title_full_unstemmed Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
title_sort Halotolerant bacteria with lipolytic activity isolated from Pilluana Salterns - San Martin
dc.creator.none.fl_str_mv Flores-Fernández, Mónica L.
Zavaleta, Amparo I.
Chávez-Hidalgo, Elizabeth L.
author Flores-Fernández, Mónica L.
author_facet Flores-Fernández, Mónica L.
Zavaleta, Amparo I.
Chávez-Hidalgo, Elizabeth L.
author_role author
author2 Zavaleta, Amparo I.
Chávez-Hidalgo, Elizabeth L.
author2_role author
author
dc.subject.none.fl_str_mv Bacterias halotolerantes
bacterias lipolíticas
suelos salinos
Salinas de Pilluana.
Halotolerant bacteria
lipolytic bacteria
salty soil
Pilluana Salterns.
topic Bacterias halotolerantes
bacterias lipolíticas
suelos salinos
Salinas de Pilluana.
Halotolerant bacteria
lipolytic bacteria
salty soil
Pilluana Salterns.
description In order to isolate halotolerant bacteria with lipolytic activity from the Pilluana Salterns in San Martin, saline soil samples were collected and cultured in 5% salt water agar containing 0,5% yeast extract. It was pre-selected 55 bacteria with different morphological characteristics, these bacteria were grown simultaneously in 5% salt water agar containing 1% tributyrin, Tween 80 and olive oil. It was selected 14 bacteria based on the ability to hydrolyze specific substrates and shown high salt tolerance rate between 0 to 25%. These bacteria grew optimally at pH 7 and 37 °C, six were Gram positive and Gram negative eight, 12 bacteria hydrolyzed tributyrin, Tween 80 and olive oil, six produced amylases and proteases. The 14 halotolerant bacteria with lipolytic activity isolated from the Pilluana salterns are promising lipase production sources and oily waste bio-treatment.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/3232
10.15381/ci.v13i2.3232
url https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/3232
identifier_str_mv 10.15381/ci.v13i2.3232
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/3232/2700
dc.rights.none.fl_str_mv Derechos de autor 2010 Mónica L. Flores-Fernández, Amparo I. Zavaleta, Elizabeth L. Chávez-Hidalgo
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2010 Mónica L. Flores-Fernández, Amparo I. Zavaleta, Elizabeth L. Chávez-Hidalgo
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica
dc.source.none.fl_str_mv Ciencia e Investigación; Vol. 13 Núm. 2 (2010); 88-92
Ciencia e Investigación; Vol. 13 No. 2 (2010); 88-92
1609-9044
1561-0861
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1863015286356049920
score 15,81155

Similares en LA Referencia