Biodiversity of Bacillus thuringiensis isolated from peruvian agroecosystem and evaluation of bioinsecticidal potential

The biological control of pests & mites on peruvian agriculture was characterized by the intensive use of chemical pesticides. However, the decrease on yield and the emergence of resistance to pesticides have obligated to farmers to increase chemical pesticide use for plague control. This ha...

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Detalles Bibliográficos
Autores: Flores P, Abad, Egúsquiza Ch., Rosa m., Alcarraz C., Mario, Woolcott H., Juan C., Benavides R, Elena, Godoy A., Juan, Huerta C., Doris, Jesus M., Yisella, Patiño G., Alejandro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Perú
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Idioma:español
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/3235
Acceso en línea:https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/3235
Access Level:acceso abierto
Palabra clave:Bacillus thuringiensis
aislamiento
Spodoptera frugiperda
toxicidad
entomotóxico.
isolation
toxicity
Spodoptera frugiperda.
Descripción
Sumario:The biological control of pests & mites on peruvian agriculture was characterized by the intensive use of chemical pesticides. However, the decrease on yield and the emergence of resistance to pesticides have obligated to farmers to increase chemical pesticide use for plague control. This has caused environmental pollution, benefical insects death, persistence chemicals residues on fruits, acute and chronic toxicity risks, moreover an increase on production costs. The objectives of this research were to isolate and to characterize B. thuringiensis native strains, and) to evaluate entomotoxic potential against Spodoptera frugiperda. Were isolated 54 natives strains of Bacillus thuringiensis from peruvian agricultural soils. Microscopic morphology showed parasporal crystals characteristic of B. thuringiensis diversity. The strains were evaluated and confirmed with biochemistry and growing characteristic. The bioassays performed on Spodoptera frugiperda showed the strain BT-UNMSM-42 had the most efficient toxicity with 39,73% mortality to 50 μg/mL and 71,93% mortality to 250 μg/mL, with standard deviation 11,30 and 9,98; respectively.