Antimicrobial efficacy of eucalyptus essential oil (Eucalyptus spp) on Escherichia coli and Staphylococcus aureus subsp. aureus strains
The objective of this study was to evaluate the in vitro antimicrobial effect of eucalyptus essential oil (Eucaliptus spp) on Escherichia coli ATCC® 11229 and Staphylococcus aureus subsp. aureus ATCC® 25904. Concentrations of 30, 60 and 90% were evaluated in dilution in 96.8% ethanol. The Minimum In...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2019 |
| Country: | Perú |
| Institution: | Universidad Nacional Mayor de San Marcos |
| Repository: | Revistas - Universidad Nacional Mayor de San Marcos |
| Language: | Spanish |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/16099 |
| Online Access: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16099 |
| Access Level: | Open access |
| Keyword: | Minimum Inhibitory Concentration; Minimum Bactericidal Concentration; MacFarland Concentración Mínima Inhibitoria; Concentración Mínima Bactericida; MacFarland |
| Summary: | The objective of this study was to evaluate the in vitro antimicrobial effect of eucalyptus essential oil (Eucaliptus spp) on Escherichia coli ATCC® 11229 and Staphylococcus aureus subsp. aureus ATCC® 25904. Concentrations of 30, 60 and 90% were evaluated in dilution in 96.8% ethanol. The Minimum Inhibitory Concentration (MIC) was determined by the broth microdilution method. The bacterial inoculum was standardized to 0.5 of the MacFarland scale in spectrophotometer, obtaining as a result that the concentrations of 60 and 90% did not present turbidity. The Minimum Bactericidal Concentration (MBC) determined in Mueller-Hinton agar was present from the 60% concentration for the two strains under study. The antimicrobial susceptibility test indicated that all concentrations showed antimicrobial sensitivity and that the concentrations at 30 and 60% were not significantly different, presenting halos of inhibition of 10.25 mm and 10.65 mm in the concentration of 30% for the strains of Escherichia coli and Staphylococcus. aureus subsp. aureus, respectively. On the other hand, the results for the strain S. aureus subsp. aureus showed that concentrations at 60% and 90% formed larger diameter halos than the E. coli strain. |
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