Physico-chemical characterization of a lectin from pisum sativum “arveja” seeds and antifungical effect on fusarium oxysporum
By following previous method proceedings, a lectin was purified and characterized from Pisum sativum L. (Pea, Papilionaceae, Leguminosae) seeds through combination of one Sephadex G-75 gel filtration chromatography and a reverse phase HPLC in -Bondapack C18 column. The analysis in bidimentional elec...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Perú |
| Institución: | Universidad Católica de Santa María |
| Repositorio: | Revistas - Universidad Católica de Santa María |
| Idioma: | español |
| OAI Identifier: | oai:ojs.revistas.ucsm.edu.pe:article/190 |
| Acceso en línea: | https://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/190 |
| Access Level: | acceso abierto |
| Palabra clave: | Pisum sativum lectina actividad hermaglutinante actividad antifúngica |
| Sumario: | By following previous method proceedings, a lectin was purified and characterized from Pisum sativum L. (Pea, Papilionaceae, Leguminosae) seeds through combination of one Sephadex G-75 gel filtration chromatography and a reverse phase HPLC in -Bondapack C18 column. The analysis in bidimentional electrophoresis (2D) showed a protein spot of 14 KDa and an isoelectrical point of 7.5. The molecular weight of 14 kDa was confirmed by mass spectrometry MALDI -TOF in 14 662.0 Da. The aminoacid composition showed an hydrophobical protein with basic features and bioinformatic analysis of the N-terminal sequence showed homology with other vegetable lectins. Likewise, most outstanding physical-chemical characteristics were: the glicoprotein nature and its specificity by D- mannose and D- glucose,the Ca2+ dependence and Mn2+ in order to show hemnogglutinan activity. The study of the fungicidal activity of V-2 fraction with the hemoglutinan activity over the Fusarium oxysporum reveals that lectin V-2 delays development of the fungi and inhibits its growing. |
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