Caracterización fenotípica y genotípica de factores de virulencia de cepas de Escherichia coli uropatógena productora de carbapenemasa
Introduction. Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs). Its carbapenem-resistant form (UPEC-rC) harbors specific virulence factors that further limit therapeutic options. Objective. Identify virulence factors in UPEC-rC isolates recovered from uri...
| Autores: | , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2025 |
| País: | Perú |
| Recursos: | Universidad Nacional Mayor de San Marcos |
| Repositório: | Revistas - Universidad Nacional Mayor de San Marcos |
| Idioma: | espanhol |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/31563 |
| Acesso em linha: | https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/31563 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Uropathogenic Escherichia coli Carbapenem-Resistant Enterobacteriaceae Virulence Factors Escherichia coli Uropatógena Farmacorresistencia Bacteriana Factores de virulencia Enterobacteriaceae Resistentes a los Carbapenémicos |
| Resumo: | Introduction. Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs). Its carbapenem-resistant form (UPEC-rC) harbors specific virulence factors that further limit therapeutic options. Objective. Identify virulence factors in UPEC-rC isolates recovered from urine samples of patients with UTIs in Lima, Peru. Methods. We conducted a descriptive observational study including 58 UPEC-rC isolates. Virulence factors were analyzed in the laboratory: phenotypically (hemolysin on blood agar, P fimbriae by erythrocyte hemagglutination and biofilms production using the microtiter plate method) and genotypically (α-hly, papGII, aer, cnf1, sfa/focDE) by PCR. Results. 28 out of 58 isolates exhibited at least one virulence factor. Genotypically, aer gene was the most frequent (34,5%), while phenotypically, biofilm formation predominated (17,2%). Conclusion. The frequency of aer gen and biofilm production suggests a possible relationship between carbapenem resistance and the attenuation of pathogenic mechanisms. |
|---|