Histopathological study of the effects of administering heme iron and ferrous sulfate with vitamin C in rat liver and brain
Objective: To determine the effect exerted by the administration of heme iron and ferrous sulfate with vitamin C in rat liver and brain. Materials and methods: The study used Holtzman albino rats housed in a bioterium with a temperature of 22 ± 2 °C, humidity between 50 and 70 %, and 12 hours of lig...
| Autores: | , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Perú |
| Recursos: | Universidad de San Martín de Porres |
| Repositorio: | Horizonte médico |
| Idioma: | español |
| OAI Identifier: | oai:horizontemedico.usmp.edu.pe:article/972 |
| Acesso em linha: | https://horizontemedico.usmp.edu.pe/index.php/horizontemed/article/view/972 |
| Access Level: | acceso abierto |
| Palavra-chave: | Hierro Hemo Ácido ascórbico Toxicidad Iron Heme Ascorbic acid Toxicity |
| Resumo: | Objective: To determine the effect exerted by the administration of heme iron and ferrous sulfate with vitamin C in rat liver and brain. Materials and methods: The study used Holtzman albino rats housed in a bioterium with a temperature of 22 ± 2 °C, humidity between 50 and 70 %, and 12 hours of light and 12 hours of darkness. They received elemental iron 4.0 mg/kg b.w. as heme iron or ferrous sulfate + vitamin C 10 mg for seven days, at which time they were sacrificed, and blood, liver and brain were extracted. Histological sections were made and treated with hematoxylin-eosin for microscopic observation, and serum antioxidant capacity was measured. Results: The brains of the rats treated with heme iron and ferrous sulfate + vitamin C did not undergo significant changes, while the histological sections of the livers of the rats treated with heme iron showed a parenchyma without polar distribution, some nuclei lacking cytoplasm and numerous Küpffer cells at the sinusoidal level. In contrast, the rats treated with ferrous sulfate + vitamin C had a significantly deteriorated hepatic parenchyma, some areas with loose nuclei without cytoplasm and others with disappeared cytoplasmic membranes. In addition, in some areas, the liver parenchyma was homogenized. Conclusions: The brains of the rats treated with heme iron and those with ferrous sulfate + vitamin C did not practically undergo any change. In contrast, the liver of the rats treated with ferrous sulfate + vitamin C had greater liver damage than those treated with heme iron. |
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