Detección molecular del virus del distemper canino en casos clínicos de caninos domésticos no vacunados y evaluación de factores de riesgo

The canine distemper (CD) is a viral infection caused by a Morbillivirus of the family Paramyxoviridae. It can generate digestive, respiratory, and nervous symptomatology, depending on the infecting strain. This study was designed to detect the CD virus through the RT-PCR technique and perform the a...

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Detalles Bibliográficos
Autores: Soto R., Andrea, Luna E., Luis R., Rosadio A., Raúl, Maturrano H., Lenin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Perú
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Idioma:español
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/14744
Acceso en línea:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14744
Access Level:acceso abierto
Palabra clave:distemper canino
detección molecular
PCR
canine distemper
molecular detection
nucleoprotein
Descripción
Sumario:The canine distemper (CD) is a viral infection caused by a Morbillivirus of the family Paramyxoviridae. It can generate digestive, respiratory, and nervous symptomatology, depending on the infecting strain. This study was designed to detect the CD virus through the RT-PCR technique and perform the analysis of the data collected to have a current view of the behavior of this virus in Lima, Peru. Whole blood samples from 52 unvaccinated canines with clinical signs compatible with canine distemper were collected between June 2012 and January 2015. The nucleoprotein of 287 bp was successfully amplified in 32.7% (17/52) of the samples analyzed. In addition, it was found a greater detection of the virus, but not significative, in individuals that manifested systemic clinical signs (respiratory and digestive signs vs nervous) and within the age range of 1.5-4 months.